Fluorescent nanoparticles using hyaluronic acid-histidine-based manganese dioxide for hydrogen peroxide imaging in inflammatory macrophages

IF 2.8 4区工程技术 Q2 POLYMER SCIENCE

Macromolecular Research Pub Date : 2024-11-12 DOI:10.1007/s13233-024-00337-8

Ji Yeon Hong, Ji Sun Park, Kyeongsoon Park

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引用次数: 0

Abstract

The presence of excessive hydrogen peroxide (H₂O₂) in the body plays a key role in inflammatory diseases. Among the various methods available, fluorescent nanoparticles have been used to detect H₂O₂ in living cells. Herein, the design of CD44-targetable fluorescent nanoparticles is presented for intracellular H₂O₂ imaging in inflammatory macrophages. Accordingly, CD44-targetable hyaluronic acid-histidine (HA–His) conjugates were sequentially reacted with permanganate and fluorescein (FL) to synthesize HA–His/MnO₂/FL nanoparticles. These prepared HA–His/MnO₂/FL nanoparticles exhibited good dispersibility in water and 10% fetal bovine serum solution. Owing to their rapid responsiveness to H₂O₂, the HA–His/MnO₂/FL nanoparticles showed a good linear relationship with H₂O₂ concentration (0–100 μM). Moreover, the CD44-targetable HA–His/MnO₂/FL nanoparticles were readily internalized in lipopolysaccharide-induced inflammatory macrophages, allowing successful imaging of intracellular H₂O₂ in inflammatory macrophages.

Graphical Abstract

The HA-His/MnO₂/FL nanoparticles with H₂O₂ responsiveness were readily internalized into activated macrophages, allowing successful intracellular H₂O₂ imaging in inflammatory macrophages

Abstract Image

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荧光纳米颗粒使用透明质酸组氨酸为基础的二氧化锰过氧化氢成像炎症巨噬细胞

体内过量过氧化氢（H2O2）的存在在炎症性疾病中起着关键作用。在各种可用的方法中，荧光纳米颗粒已被用于检测活细胞中的H2O2。本文提出了cd44靶向荧光纳米颗粒的设计，用于炎症巨噬细胞内H2O2成像。因此，cd44靶向透明质酸-组氨酸（HA-His）偶联物依次与高锰酸盐和荧光素（FL）反应，合成HA-His /MnO2/FL纳米颗粒。制备的HA-His /MnO2/FL纳米颗粒在水和10%胎牛血清溶液中具有良好的分散性。HA-His /MnO2/FL纳米粒子对H2O2的响应速度快，与H2O2浓度（0 ~ 100 μM）呈良好的线性关系。此外，可靶向cd44的HA-His /MnO2/FL纳米颗粒很容易内化到脂多糖诱导的炎性巨噬细胞中，从而成功成像炎性巨噬细胞内的H2O2。具有H2O2响应性的HA-His/MnO2/FL纳米颗粒很容易内化到活化的巨噬细胞中，从而成功地在炎症性巨噬细胞中进行细胞内H2O2成像

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来源期刊

Macromolecular Research 工程技术-高分子科学

CiteScore

4.70

自引率

8.30%

发文量

100

审稿时长

1.3 months

期刊介绍： Original research on all aspects of polymer science, engineering and technology, including nanotechnology Presents original research articles on all aspects of polymer science, engineering and technology Coverage extends to such topics as nanotechnology, biotechnology and information technology The English-language journal of the Polymer Society of Korea Macromolecular Research is a scientific journal published monthly by the Polymer Society of Korea. Macromolecular Research publishes original researches on all aspects of polymer science, engineering, and technology as well as new emerging technologies using polymeric materials including nanotechnology, biotechnology, and information technology in forms of Articles, Communications, Notes, Reviews, and Feature articles.