Observation of Ice-Like Two-Dimensional Flakes on Self-Assembled Protein Monolayer without Nanoconfinement under Ambient Conditions

IF 26.6 1区材料科学 Q1 Engineering

Nano-Micro Letters Pub Date : 2025-03-14 DOI:10.1007/s40820-025-01689-1

Wuxian Peng, Linbo Li, Xiyue Bai, Ping Yi, Yu Xie, Lejia Wang, Wei Du, Tao Wang, Jian-Qiang Zhong, Yuan Li

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Abstract

Directly correlating the morphology and composition of interfacial water is vital not only for studying water icing under critical conditions but also for understanding the role of protein–water interactions in bio-relevant systems. In this study, we present a model system to study two-dimensional (2D) water layers under ambient conditions by using self-assembled monolayers (SAMs) supporting the physisorption of the Cytochrome C (Cyt C) protein layer. We observed that the 2D island-like water layers were uniformly distributed on the SAMs as characterized by atomic force microscopy, and their composition was confirmed by nano-atomic force microscopy-infrared spectroscopy and Raman spectroscopy. In addition, these 2D flakes could grow under high-humidity conditions or melt upon the introduction of a heat source. The formation of these flakes is attributed to the activation energy for water desorption from the Cyt C being nearly twofold high than that from the SAMs. Our results provide a new and effective method for further understanding the water–protein interactions.

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环境条件下无纳米约束自组装蛋白单层上冰状二维薄片的观察

直接关联界面水的形态和组成不仅对于研究临界条件下的水结冰，而且对于理解蛋白质-水相互作用在生物相关系统中的作用至关重要。在这项研究中，我们提出了一个模型系统，通过使用支持细胞色素C （Cyt C）蛋白层物理吸附的自组装单层（sam）来研究环境条件下的二维（2D）水层。通过原子力显微镜对其进行了表征，发现其表面均匀分布着二维岛状水层，并通过纳米原子力显微镜-红外光谱和拉曼光谱对其组成进行了验证。此外，这些二维薄片可以在高湿条件下生长或在引入热源时熔化。这些薄片的形成是由于Cyt C解吸水的活化能几乎是SAMs的两倍。我们的研究结果为进一步了解水蛋白相互作用提供了一种新的有效方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nano-Micro Letters NANOSCIENCE & NANOTECHNOLOGY-MATERIALS SCIENCE, MULTIDISCIPLINARY

CiteScore

32.60

自引率

4.90%

发文量

981

审稿时长

1.1 months

期刊介绍： Nano-Micro Letters is a peer-reviewed, international, interdisciplinary, and open-access journal published under the SpringerOpen brand. Nano-Micro Letters focuses on the science, experiments, engineering, technologies, and applications of nano- or microscale structures and systems in various fields such as physics, chemistry, biology, material science, and pharmacy.It also explores the expanding interfaces between these fields. Nano-Micro Letters particularly emphasizes the bottom-up approach in the length scale from nano to micro. This approach is crucial for achieving industrial applications in nanotechnology, as it involves the assembly, modification, and control of nanostructures on a microscale.