{"title":"A smartphone-based portable electrochemical sensor enabled ultrasensitive detection of paclitaxel in serum and injection samples","authors":"Ruo-Yu Yang, Jin-Hua Wang, Zi-Wei Yu, Yun-Ting Chen, Mei-Juan Wu, Pin-Fang Huang, Meng-Meng Liu","doi":"10.1007/s00604-025-07085-1","DOIUrl":null,"url":null,"abstract":"<div><p>A point-of-care testing platform, consisting of smartphone, miniature electrochemical workstation, and screen-printed carbon electrode (SPCE) modified by gold nanoparticle (AuNPs) and multi-walled carbon nanotubes (MWCNTs), is fabricated for the ultrasensitive detection of paclitaxel (PTX) in human serum and injection solution. To enhance conductivity of the sensing system, MWCNTs concentration and AuNPs electrodeposition time were optimized. The AuNPs/MWCNTs effectively increase the working electrode area of SPCE by a factor of 1.46, contributing to improved electrochemical performance. The steps of electrode surface modification and the characterization of AuNPs/MWCNTs/SPCE were investigated by differential pulse voltammetry, impedance spectroscopy, X-ray photoelectron spectroscopy, and scanning electron microscopy. The sensor shows good linearity between current response and PTX concentration in 0.2 M phosphate buffer at pH = 7.4 (0.05–10 μM, with a limit of detection (LOD) of 1.7 nM) and human serum (0.5–30 μM and a LOD of 3.6 nM). The recoveries range from 89.91 to 103.36% and 91.42 to 103.73% in human serum and injection solution, respectively, with satisfactory relative standard deviation. Moreover, the sensor has excellent stability during 8 weeks and exhibits outstanding specificity and reproducibility towards PTX detection, providing a possible option for PTX determination in practical application such as therapeutic drug monitoring and drug quality control.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3000,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchimica Acta","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00604-025-07085-1","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
A point-of-care testing platform, consisting of smartphone, miniature electrochemical workstation, and screen-printed carbon electrode (SPCE) modified by gold nanoparticle (AuNPs) and multi-walled carbon nanotubes (MWCNTs), is fabricated for the ultrasensitive detection of paclitaxel (PTX) in human serum and injection solution. To enhance conductivity of the sensing system, MWCNTs concentration and AuNPs electrodeposition time were optimized. The AuNPs/MWCNTs effectively increase the working electrode area of SPCE by a factor of 1.46, contributing to improved electrochemical performance. The steps of electrode surface modification and the characterization of AuNPs/MWCNTs/SPCE were investigated by differential pulse voltammetry, impedance spectroscopy, X-ray photoelectron spectroscopy, and scanning electron microscopy. The sensor shows good linearity between current response and PTX concentration in 0.2 M phosphate buffer at pH = 7.4 (0.05–10 μM, with a limit of detection (LOD) of 1.7 nM) and human serum (0.5–30 μM and a LOD of 3.6 nM). The recoveries range from 89.91 to 103.36% and 91.42 to 103.73% in human serum and injection solution, respectively, with satisfactory relative standard deviation. Moreover, the sensor has excellent stability during 8 weeks and exhibits outstanding specificity and reproducibility towards PTX detection, providing a possible option for PTX determination in practical application such as therapeutic drug monitoring and drug quality control.
期刊介绍：
As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.
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