Comparison between inductively coupled plasma-mass spectrometry and benchtop X-ray fluorescence performance for trace elemental exposure in rat tissues

Journal of trace elements and minerals Pub Date : 2025-03-08 DOI:10.1016/j.jtemin.2025.100229

Kolawole E. Adesina , Stefano A. Parducci , Joseph D. Brain , Ramon M. Molina , Marc Weisskopf , Aaron J. Specht

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Abstract

Background

Trace elemental toxicants induce health detriment in almost every organ system in the human body and account for a large amount of environmental and ecological environmental pollution. Traditionally, inductively coupled plasma mass spectrometry (ICP-MS) has been the gold standard for measuring elemental concentrations in biological tissues collected from toxicological and epidemiological studies. However, ICP-MS is often limited by its complexity, cost, and time-intensive nature.

Methods

This study investigates the feasibility of benchtop X-ray fluorescence (XRF) as an efficient alternative for trace elemental analysis in rat tissues, offering comparable quantification capabilities with enhanced operational simplicity. We conducted a comparative analysis using tissue samples from multiple rat organs, including stomach, eyes, and liver.

Results

The elemental concentrations of Arsenic (As), Cadmium (Cd), Copper (Cu), Manganese (Mn), and Zinc (Zn) were measured using both ICP-MS and a high-powered benchtop XRF (Epsilon 4, Malvern Panalytical). Our findings demonstrated strong linear regression correlations between the two methods: As (R² = 0.86), Cd (R² = 0.81), Cu (R² = 0.77), Mn (R² = 0.88), and Zn (R² = 0.74). The overall Pearson correlation coefficient was r = 0.95 (p ≤ 0.05), indicating high concordance between the mean concentrations obtained from ICP-MS and benchtop XRF. The median minimum detection limits for the elements were 0.12 µg/g, with specific limits for Cd (0.0042 µg/g), Cu (0.040 µg/g), Zn (0.12 µg/g), As (0.25 µg/g), and Mn (0.35 µg/g) over a 7.5-minute measurement period. Bland-Altman analysis revealed high agreement between the two methods, particularly for As, Cu, and Mn.

Conclusion

These results suggest that both ICP-MS and benchtop XRF are viable for elemental quantification in organ tissues, with benchtop XRF being more practical for low-mass samples. This study shows benchtop XRF's potential for high-throughput, accurate trace element analysis in biological samples, broadening its use in environmental and toxicological research.

Synopsis

Human and ecological tissues of varying compositions and densities can be measured effectively using benchtop X-ray fluorescence

查看原文本刊更多论文

电感耦合等离子体质谱法与台式x射线荧光法测定大鼠组织中微量元素暴露的比较

微量元素毒物对人体几乎每个器官系统都有危害，造成了大量的环境和生态环境污染。传统上，电感耦合等离子体质谱（ICP-MS）一直是测量从毒理学和流行病学研究中收集的生物组织中元素浓度的金标准。然而，ICP-MS通常受到其复杂性、成本和时间密集性的限制。方法本研究探讨了台式x射线荧光（XRF）作为大鼠组织中痕量元素分析的一种有效替代方法的可行性，该方法具有可比较的定量能力，且操作简单。我们使用来自大鼠多个器官的组织样本进行了比较分析，包括胃、眼睛和肝脏。结果采用ICP-MS和大功率台式XRF （Epsilon 4, Malvern Panalytical）同时测定了砷（As）、镉（Cd）、铜（Cu）、锰（Mn）和锌（Zn）的元素浓度。结果表明，As （R²= 0.86）、Cd （R²= 0.81）、Cu （R²= 0.77）、Mn （R²= 0.88）和Zn （R²= 0.74）之间存在较强的线性回归关系。总体Pearson相关系数r = 0.95 (p≤0.05)，表明ICP-MS测定的平均浓度与台式XRF测定的浓度高度一致。在7.5分钟的测量周期内，这些元素的中位最低检出限为0.12µg/g，其中Cd（0.0042µg/g）、Cu（0.040µg/g）、Zn（0.12µg/g）、As（0.25µg/g）和Mn（0.35µg/g）的检出限为0.12µg/g。Bland-Altman分析揭示了两种方法之间的高度一致性，特别是对于As， Cu和Mn。结论ICP-MS和台式XRF均可用于器官组织中元素的定量，台式XRF对低质量样品的定量更为实用。这项研究显示了台式XRF在高通量、精确的生物样品痕量元素分析方面的潜力，扩大了其在环境和毒理学研究中的应用。使用台式x射线荧光可以有效地测量不同成分和密度的人体和生态组织

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of trace elements and minerals Medicine and Dentistry (General), Analytical Chemistry, Environmental Science (General), Toxicology, Biochemistry, Genetics and Molecular Biology (General), Nutrition, Veterinary Science and Veterinary Medicine (General)

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审稿时长

65 days