Bioguided isolation, identification and activity evaluation of antibacterial compounds from Combretum rhodanthum Engl. & Diels

IF 2.7 3区 生物学 Q2 PLANT SCIENCES
Joseph Mbaku Ngoumou , Vincent Ngouana , Boniface Pone Kamdem , Natacha Paule Marie Ndzie , Brice Rostan Pinlap , Aubin Kamche Youbi , Paul Keilah Lunga , Rémy Bertrand Teponno , Léon Azefack Tapondjou , Fabrice Fekam Boyom
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Abstract

Infectious diseases cause over 13 million deaths worldwide annually as a result of the spread of microbial resistance. The use of medicinal plants in the treatment of infectious diseases is well documented. One such plant includes Combretum rhodanthum Engl.& Diels. (C. rhodanthum), which is traditionally used to treat skin and diarrheal infections, and gastrointestinal disorders, among others. Thus, the scientific validation of the use of C. rhodanthum in ethnomedicine is valuable. This study sought to investigate the activity-guided isolation of antibacterial compounds of C. rhodanthum. The plant was extracted with ethanol, and the resulting extract was partitioned using solvents of increasing polarity. After screening the as-prepared extract and fractions for antibacterial activity, the most active fraction (n-butanol fraction) was subjected to flash chromatography to afford eight subfractions, which were subsequently purified using column chromatography. Next, extract, fractions and compounds of C. rhodanthum were screened for antibacterial activity by microdilution method. The cytotoxicity assay was conducted on murine macrophage RAW 264.7 cells. Molecular docking studies were performed using AutoDock Vina. Herein, the ethanol extract inhibited the growth of the eleven bacterial strains tested (minimum inhibitory concentrations (MICs): 0.62 to 10 mg/mL). The fractions and subfractions showed more potent antibacterial activity with MICs and MBCs (minimum bactericidal concentrations) ranging from 1.25 to 10 mg/mL and from 0.62 to 5 mg/mL, respectively. Of the six compounds (CR1CR6) isolated from C. rhodanthum, only CR6 showed moderate bactericidal activity against a multi-resistant strain of Staphylococcus aureus. Docking studies of CR6 at the bioactive cascade of dihydropteroate synthase and UDP-N-acetylenolpyruvylglucosamine reductase have established that hydrogen bonding and optimized hydrophobic interactions stabilized the ligand at the target site. This novel contribution demonstrates the antibacterial activity of extracts and a pentacyclic triterpenoid (CR6) from C. rhodanthum, which can serve as starting points for the discovery of effective antimicrobial drugs. Nevertheless, in-depth mechanisms of action, toxicity and pharmacokinetic studies, and in vivo experiments are warranted for the successful utilization of C. rhodanthum in ethnomedicine.

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来源期刊
South African Journal of Botany
South African Journal of Botany 生物-植物科学
CiteScore
5.20
自引率
9.70%
发文量
709
审稿时长
61 days
期刊介绍: The South African Journal of Botany publishes original papers that deal with the classification, biodiversity, morphology, physiology, molecular biology, ecology, biotechnology, ethnobotany and other botanically related aspects of species that are of importance to southern Africa. Manuscripts dealing with significant new findings on other species of the world and general botanical principles will also be considered and are encouraged.
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