Technical improvement on next-generation sequencing in clinical application

IF 1.7 Q3 MEDICAL LABORATORY TECHNOLOGY

Practical Laboratory Medicine Pub Date : 2025-03-08 DOI:10.1016/j.plabm.2025.e00465

Xiaolei Xie, Weiguo Yin, Shuxia Xuan, Fuguang Li

引用次数: 0

Abstract

The next-generation sequencing (NGS) technology is currently widely utilized in clinical laboratories. This paper describes a method of technical improvement on NGS, which increases the success rates of NGS detection. This study reveals that the DNA library concentration is the highest at a molar ratio of 100:1 for the adapter to DNA. The self-revised method for adaptor ligation can effectively improve the success rate of DNA library, particularly in manual operations. Additionally, the modified pooling method, which incorporates various DNA fragment sizes for different NGS projects, proves beneficial for medical laboratory applications.

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来源期刊

Practical Laboratory Medicine Health Professions-Radiological and Ultrasound Technology

CiteScore

3.50

自引率

0.00%

发文量

审稿时长

7 weeks

期刊介绍： Practical Laboratory Medicine is a high-quality, peer-reviewed, international open-access journal publishing original research, new methods and critical evaluations, case reports and short papers in the fields of clinical chemistry and laboratory medicine. The objective of the journal is to provide practical information of immediate relevance to workers in clinical laboratories. The primary scope of the journal covers clinical chemistry, hematology, molecular biology and genetics relevant to laboratory medicine, microbiology, immunology, therapeutic drug monitoring and toxicology, laboratory management and informatics. We welcome papers which describe critical evaluations of biomarkers and their role in the diagnosis and treatment of clinically significant disease, validation of commercial and in-house IVD methods, method comparisons, interference reports, the development of new reagents and reference materials, reference range studies and regulatory compliance reports. Manuscripts describing the development of new methods applicable to laboratory medicine (including point-of-care testing) are particularly encouraged, even if preliminary or small scale.