Potential of size-exclusion chromatography and asymmetric flow field-flow fractionation in separation and characterization of plant polysaccharides

IF 4 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography A Pub Date : 2025-03-09 DOI:10.1016/j.chroma.2025.465862

Leena Pitkänen

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引用次数: 0

Abstract

Size-exclusion chromatography (SEC) and asymmetric flow field-flow fractionation (AF4) are elution-based techniques for separation and characterization of (bio)macromolecules including plant polysaccharides. These two techniques separate the macromolecules according to their hydrodynamic volume in solution. The primary reason for the separation of polysaccharides with a dispersed nature is the need for accurate information on the molar mass distribution (MMD), which cannot be obtained without the separation of macromolecular species into narrowly distributed fractions. Depending on the detectors coupled to the separation, other information such as size, branching, and conformation can also be obtained across the separated fractions. This review summarizes the SEC and AF4 methodology used for separation and characterization of plant polysaccharides with industrial and/or nutritional importance. The key differences between the two techniques are highlighted and recommendations are given for method selection.

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尺寸排阻色谱法和非对称流场-流动分馏法在植物多糖的分离和表征中的潜力

粒径排除色谱（SEC）和不对称流场-流分馏法（AF4）是一种基于洗脱的分离和表征（生物）大分子（包括植物多糖）的技术。这两种技术根据大分子在溶液中的流体力学体积分离大分子。分离具有分散性质的多糖的主要原因是需要准确的摩尔质量分布（MMD）信息，如果没有将大分子物种分离成狭窄分布的馏分，就无法获得这些信息。根据与分离相耦合的检测器，还可以获得分离分数的其他信息，如大小、分支和构象。本文综述了用于分离和表征具有工业和/或营养重要性的植物多糖的SEC和AF4方法。强调了两种技术之间的关键区别，并给出了方法选择的建议。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Chromatography A 化学-分析化学

CiteScore

7.90

自引率

14.60%

发文量

742

审稿时长

45 days

期刊介绍： The Journal of Chromatography A provides a forum for the publication of original research and critical reviews on all aspects of fundamental and applied separation science. The scope of the journal includes chromatography and related techniques, electromigration techniques (e.g. electrophoresis, electrochromatography), hyphenated and other multi-dimensional techniques, sample preparation, and detection methods such as mass spectrometry. Contributions consist mainly of research papers dealing with the theory of separation methods, instrumental developments and analytical and preparative applications of general interest.