Acupuncture ameliorates inflammation by regulating gut microbiota in acute ischemic stroke

IF 2.9 Q3 NEUROSCIENCES

IBRO Neuroscience Reports Pub Date : 2025-02-17 DOI:10.1016/j.ibneur.2025.02.007

Haoyue Yan , Yini Hua , Jinxia Ni , Xiaona Wu , Jingni Xu , Ziniu Zhang , Juwei Dong , Zhihao Xiong , Lei Yang , Hongwei Yuan

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Abstract

Background

Acute ischemic stroke(AIS) is a major life-threatening disease.Some studies have found that AIS may be related to gut flora and immune responses. Acupuncture is used widely in the treatment of AIS. However its relevant mechanism is unclear enough. Therefore, in this study, we wanted to confirm that acupuncture was treating AIS through gut flora and immune response.

Methods

We randomly divided 18 rats into equal three groups, including Sham, Middle Cerebral Artery Occlusion (MCAO) and Acupuncture.Rats in the Acupuncture group for a continuous period of three days after surgery. Neurological deficits were assessed using Longa’s method, and detection of intestinal flora by 16s rRNA gene sequencing, determination of SCFAs by gas chromatography-mass spectrometry, detection of HDAC and inflammatory cytokines by elisa assay, detection of Th17 and Treg cells by flow cytometry and, observation of pathological and morphological changes in brain and colon tissues by HE staining.

Results

Acupuncture improved the degree of impaired neurological function in MCAO rats and regulated the type and abundance of intestinal bacteria, increased SCFAs of MCAO rats, decreased HDAC1 and HDAC2, modulated the Th17/Treg imbalance, reduced the level of inflammatory factors in the peripheral blood and altered the pathology of the intestine and brain.

Conclusion

Acupuncture repaired neurologic deficits after AIS and may be associated with an immune-inflammatory response mediated by gut microbiota.

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针刺通过调节急性缺血性脑卒中患者的肠道微生物群来改善炎症

急性缺血性脑卒中（acute ischemic stroke， AIS）是一种严重危及生命的疾病。一些研究发现AIS可能与肠道菌群和免疫反应有关。针灸在AIS的治疗中应用广泛。然而，其相关机制尚不清楚。因此，在本研究中，我们想确认针灸通过肠道菌群和免疫反应治疗AIS。方法将18只大鼠随机分为假手术组、大脑中动脉闭塞组和针刺组。针刺组大鼠术后连续治疗3天。采用龙格法评估神经功能缺损，16s rRNA基因测序检测肠道菌群，气相色谱-质谱法检测SCFAs， elisa法检测HDAC和炎性细胞因子，流式细胞术检测Th17和Treg细胞，HE染色观察脑和结肠组织病理形态学变化。结果针刺可改善MCAO大鼠神经功能受损程度，调节肠道细菌种类和丰度，增加MCAO大鼠SCFAs，降低HDAC1和HDAC2，调节Th17/Treg失衡，降低外周血炎症因子水平，改变肠、脑病理。结论针刺可修复AIS后的神经功能缺损，可能与肠道菌群介导的免疫炎症反应有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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