Functional Bacterial Cellulose-Based MXene (Ti3C2T x ) Electronic-Skin Patch for Accelerated Healing and Monitoring.

IF 5 Q1 ENGINEERING, BIOMEDICAL
BME frontiers Pub Date : 2025-03-11 eCollection Date: 2025-01-01 DOI:10.34133/bmef.0109
Saliha Nur lIhan, Bahar Akyuz Yilmaz, Fatih Ciftci
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引用次数: 0

Abstract

Objective: This study aims to develop and characterize electroactive hydrogels based on reduced bacterial cellulose (BC) and Ti3C2T x -MXene for their potential application in wound healing and real-time monitoring. Impact Statement: The integration of Ti3C2T x -MXene into BC matrices represents a novel approach to creating multifunctional hydrogels that combine biocompatibility, electrical conductivity, and mechanical durability. These properties make the hydrogels promising candidates for advanced wound care and real-time monitoring applications. Introduction: Wound healing requires materials that support cell growth, promote tissue regeneration, and enable real-time monitoring. MXenes, a class of 2-dimensional materials, offer unique electrical and mechanical properties, making them suitable for biomedical applications. This study explores the integration of Ti3C2T x -MXene with BC, a biopolymer known for its excellent biocompatibility and mechanical strength, to create electroactive composite hydrogel films for advanced wound care. Methods: Ti3C2T x -MXene was synthesized by etching Ti3AlC2 with hydrofluoric acid and integrated into BC pellicles produced by Gluconacetobacter xylinum. The composite hydrogel films underwent characterization through x-ray diffraction (XRD), x-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA) to determine structural, chemical, and thermal properties. Mechanical testing assessed tensile and compressive strengths. Biological assessments, including cell viability, hemolysis rate, and protein expression, evaluated biocompatibility and regenerative potential. Results: XRD confirmed the crystallographic structure of MXene and BC composite film. XPS and FTIR validated the successful incorporation of MXene into the film matrix. Composite hydrogel films demonstrated a tensile strength of 3.5 MPa and a compressive strength of 4.2 MPa. TGA showed stability up to 350 °C, and the electrical conductivity reached 9.14 × 10-4 S/m, enabling real-time monitoring capabilities. Cell viability exceeded 95%, with a hemolysis rate below 2%. Protein expression studies revealed the ability to promote skin regeneration through collagen I, K10, K5, and filaggrin expression. Conclusion: The BC/MXene composite hydrogel films exhibit important potential as electronic-skin patches for accelerating wound healing and enabling real-time monitoring. Their unique combination of mechanical durability, electrical conductivity, and biocompatibility highlights their promise for advanced wound care applications.

功能性细菌纤维素基MXene (ti3c2tx)电子皮肤贴片加速愈合和监测。
目的:研究基于还原细菌纤维素(BC)和ti3c2tx -MXene的电活性水凝胶,研究其在伤口愈合和实时监测方面的潜在应用。影响声明:将Ti3C2T x -MXene集成到BC基质中代表了一种创造多功能水凝胶的新方法,该水凝胶结合了生物相容性、导电性和机械耐久性。这些特性使水凝胶成为高级伤口护理和实时监测应用的有希望的候选者。简介:伤口愈合需要支持细胞生长、促进组织再生和实时监测的材料。MXenes是一类二维材料,具有独特的电气和机械性能,适用于生物医学应用。本研究探讨了Ti3C2T x -MXene与BC(一种以其优异的生物相容性和机械强度而闻名的生物聚合物)的整合,以创建用于高级伤口护理的电活性复合水凝胶膜。方法:用氢氟酸蚀刻Ti3AlC2合成ti3c2tx -MXene,并将其整合到由xylinum糖醋杆菌生产的BC膜中。通过x射线衍射(XRD)、x射线光电子能谱(XPS)、傅里叶变换红外光谱(FTIR)和热重分析(TGA)对复合水凝胶膜进行表征,确定其结构、化学和热性能。机械测试评估拉伸和抗压强度。生物评估包括细胞活力、溶血率和蛋白表达,评估生物相容性和再生潜力。结果:XRD证实了MXene和BC复合膜的晶体结构。XPS和FTIR验证了MXene与薄膜基质的成功结合。复合水凝胶膜的抗拉强度为3.5 MPa,抗压强度为4.2 MPa。TGA在350℃下稳定,电导率达到9.14 × 10-4 S/m,具有实时监测能力。细胞存活率超过95%,溶血率低于2%。蛋白表达研究揭示了通过胶原I、K10、K5和聚丝蛋白表达促进皮肤再生的能力。结论:BC/MXene复合水凝胶膜作为电子皮肤贴片具有促进伤口愈合和实时监测的重要潜力。其独特的机械耐久性、导电性和生物相容性的组合突出了其在高级伤口护理应用中的前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.10
自引率
0.00%
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审稿时长
16 weeks
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