{"title":"Exosome-Derived miR-148a-3p Protect against Tumor Proliferation and Metastasis of Esophageal Squamous Carcinoma.","authors":"Jingting Zhang, Takeshi Toyozumi, Masayuki Kano, Yasunori Matsumoto, Ryota Otsuka, Nobufumi Sekino, Tadashi Shiraishi, Koichiro Okada, Toshiki Kamata, Shinichiro Iida, Hiroki Morishita, Tenshi Makiyama, Yuri Nishioka, Masanari Yamada, Masaya Uesato, Koichi Hayano, Akira Nakano, Hisahiro Matsubara","doi":"10.1159/000544987","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Esophageal squamous cell carcinoma (ESCC) is one of the most serious malignancies worldwide, seriously affecting the survival and living quality of patients. Abnormal expression of microRNAs plays a pivotal role in the development and progression of tumor, while exosomes are usually involved in some biological functions as tools for the delivery of intercellular substances, including miRNAs. The purpose of our study was to investigate the impact of exosome miR-148a-3p expression on ESCC.</p><p><strong>Methods: </strong>qRT-PCR was used to examine the relative expression of miR-148a-3p in plasma exosomes and in vitro cells. Cell proliferation ability was tested by CCK-8; the migration and invasion function were tested using Transwell assay. The overall survival (OS) and cancer-specific survival rate of ESCC patients were calculated using the Kaplan-Meier method.</p><p><strong>Results: </strong>The expression of exosomal miR-148a-3p in ESCC patients' plasma can clearly distinguish the survival rate, and the OS rate with high expression of exosomal miR-148a-3p is clearly higher than the patients with low expression. In cell function tests, the miR-148a-3p expression of ESCC cell lines was lower than in control group. After transfecting miR-148a-3p mimic, the proliferation, migration, and invasion ability of experimental group was reduced than the control group.</p><p><strong>Conclusions: </strong>Above experimental results explained that miR-148a-3p showed significant tumor inhibition function both in serum exosomes of ESCC patients and in functional tests of cancer cell lines in vitro, suggesting that exosome miR-148a-3p can inhibit tumor progression and has the potential to be used as an indicator of clinical ESCC diagnosis and prognosis.</p>","PeriodicalId":19497,"journal":{"name":"Oncology","volume":" ","pages":"1-9"},"PeriodicalIF":2.5000,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Oncology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1159/000544987","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ONCOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Esophageal squamous cell carcinoma (ESCC) is one of the most serious malignancies worldwide, seriously affecting the survival and living quality of patients. Abnormal expression of microRNAs plays a pivotal role in the development and progression of tumor, while exosomes are usually involved in some biological functions as tools for the delivery of intercellular substances, including miRNAs. The purpose of our study was to investigate the impact of exosome miR-148a-3p expression on ESCC.
Methods: qRT-PCR was used to examine the relative expression of miR-148a-3p in plasma exosomes and in vitro cells. Cell proliferation ability was tested by CCK-8; the migration and invasion function were tested using Transwell assay. The overall survival (OS) and cancer-specific survival rate of ESCC patients were calculated using the Kaplan-Meier method.
Results: The expression of exosomal miR-148a-3p in ESCC patients' plasma can clearly distinguish the survival rate, and the OS rate with high expression of exosomal miR-148a-3p is clearly higher than the patients with low expression. In cell function tests, the miR-148a-3p expression of ESCC cell lines was lower than in control group. After transfecting miR-148a-3p mimic, the proliferation, migration, and invasion ability of experimental group was reduced than the control group.
Conclusions: Above experimental results explained that miR-148a-3p showed significant tumor inhibition function both in serum exosomes of ESCC patients and in functional tests of cancer cell lines in vitro, suggesting that exosome miR-148a-3p can inhibit tumor progression and has the potential to be used as an indicator of clinical ESCC diagnosis and prognosis.
期刊介绍：
Although laboratory and clinical cancer research need to be closely linked, observations at the basic level often remain removed from medical applications. This journal works to accelerate the translation of experimental results into the clinic, and back again into the laboratory for further investigation. The fundamental purpose of this effort is to advance clinically-relevant knowledge of cancer, and improve the outcome of prevention, diagnosis and treatment of malignant disease. The journal publishes significant clinical studies from cancer programs around the world, along with important translational laboratory findings, mini-reviews (invited and submitted) and in-depth discussions of evolving and controversial topics in the oncology arena. A unique feature of the journal is a new section which focuses on rapid peer-review and subsequent publication of short reports of phase 1 and phase 2 clinical cancer trials, with a goal of insuring that high-quality clinical cancer research quickly enters the public domain, regardless of the trial’s ultimate conclusions regarding efficacy or toxicity.
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