{"title":"Measurement of Aqueous Humor Viscosity in an Experimental Rabbit Model With Corneal Neovascularization.","authors":"Dong Eun Kim, Do Young Park, Jong Chul Han","doi":"10.1167/tvst.14.3.9","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>This study investigated the relationship between aqueous humor (AH) viscosity and vascular endothelial growth factor-A (VEGF-A) in a corneal neovascularization (CNV) model.</p><p><strong>Methods: </strong>Ten female New Zealand rabbits were divided into two groups (n = 5 each). CNV was induced by alkaline burns on the right corneas of group B, whereas group A underwent a sham procedure. After 14 days, at least 150 µL of AH was extracted from both eyes. VEGF-A concentration was measured using ELISA, and AH viscosity was determined using a viscometer. Correlations between VEGF-A concentration, total protein concentration, and AH viscosity were assessed.</p><p><strong>Results: </strong>VEGF-A concentration was significantly elevated in CNV-induced eyes than in noninduced eyes (6029.06 ± 7116.50 pg/mL vs. 115.63 ± 33.19 pg/mL, P < 0.01). Total protein concentration was also elevated in CNV-induced eyes (11.66 ± 9.86 mg/mL) than in noninduced eyes (0.69 ± 0.06 mg/mL, P < 0.01), and correlated positively with VEGF-A (r = 0.84, P < 0.01). AH viscosity was significantly increased in CNV-induced eyes (1.82 ± 1.28 mPa-s) compared to noninduced eyes (1.05 ± 0.01 mPa-s, P < 0.01) and correlated strongly with VEGF-A concentration in CNV-induced eyes (r = 1.00, p = 0.02).</p><p><strong>Conclusions: </strong>AH viscosity positively correlated with VEGF-A concentration, particularly in CNV-induced eyes with elevated VEGF-A levels.</p><p><strong>Translational relevance: </strong>The correlation between VEGF-A levels and AH viscosity in an experimental model of corneal neovascularization suggests that AH viscosity could serve as a biomarker for predicting IOP or surgical outcomes in conditions like neovascular glaucoma.</p>","PeriodicalId":23322,"journal":{"name":"Translational Vision Science & Technology","volume":"14 3","pages":"9"},"PeriodicalIF":2.6000,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11918026/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Translational Vision Science & Technology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1167/tvst.14.3.9","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"OPHTHALMOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Purpose: This study investigated the relationship between aqueous humor (AH) viscosity and vascular endothelial growth factor-A (VEGF-A) in a corneal neovascularization (CNV) model.
Methods: Ten female New Zealand rabbits were divided into two groups (n = 5 each). CNV was induced by alkaline burns on the right corneas of group B, whereas group A underwent a sham procedure. After 14 days, at least 150 µL of AH was extracted from both eyes. VEGF-A concentration was measured using ELISA, and AH viscosity was determined using a viscometer. Correlations between VEGF-A concentration, total protein concentration, and AH viscosity were assessed.
Results: VEGF-A concentration was significantly elevated in CNV-induced eyes than in noninduced eyes (6029.06 ± 7116.50 pg/mL vs. 115.63 ± 33.19 pg/mL, P < 0.01). Total protein concentration was also elevated in CNV-induced eyes (11.66 ± 9.86 mg/mL) than in noninduced eyes (0.69 ± 0.06 mg/mL, P < 0.01), and correlated positively with VEGF-A (r = 0.84, P < 0.01). AH viscosity was significantly increased in CNV-induced eyes (1.82 ± 1.28 mPa-s) compared to noninduced eyes (1.05 ± 0.01 mPa-s, P < 0.01) and correlated strongly with VEGF-A concentration in CNV-induced eyes (r = 1.00, p = 0.02).
Conclusions: AH viscosity positively correlated with VEGF-A concentration, particularly in CNV-induced eyes with elevated VEGF-A levels.
Translational relevance: The correlation between VEGF-A levels and AH viscosity in an experimental model of corneal neovascularization suggests that AH viscosity could serve as a biomarker for predicting IOP or surgical outcomes in conditions like neovascular glaucoma.
期刊介绍：
Translational Vision Science & Technology (TVST), an official journal of the Association for Research in Vision and Ophthalmology (ARVO), an international organization whose purpose is to advance research worldwide into understanding the visual system and preventing, treating and curing its disorders, is an online, open access, peer-reviewed journal emphasizing multidisciplinary research that bridges the gap between basic research and clinical care. A highly qualified and diverse group of Associate Editors and Editorial Board Members is led by Editor-in-Chief Marco Zarbin, MD, PhD, FARVO.
The journal covers a broad spectrum of work, including but not limited to:
Applications of stem cell technology for regenerative medicine,
Development of new animal models of human diseases,
Tissue bioengineering,
Chemical engineering to improve virus-based gene delivery,
Nanotechnology for drug delivery,
Design and synthesis of artificial extracellular matrices,
Development of a true microsurgical operating environment,
Refining data analysis algorithms to improve in vivo imaging technology,
Results of Phase 1 clinical trials,
Reverse translational ("bedside to bench") research.
TVST seeks manuscripts from scientists and clinicians with diverse backgrounds ranging from basic chemistry to ophthalmic surgery that will advance or change the way we understand and/or treat vision-threatening diseases. TVST encourages the use of color, multimedia, hyperlinks, program code and other digital enhancements.
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