Assessment of Palatine Tonsil Testing by ELISA for Chronic Wasting Disease Detection in Free-Ranging Mule Deer (Odocoileus hemionus).

IF 1.2 4区农林科学 Q3 VETERINARY SCIENCES

Journal of Wildlife Diseases Pub Date : 2025-04-01 DOI:10.7589/JWD-D-24-00161

Marguerite D Johnson, Jennifer L Malmberg, Jaqueline P Kurz, Jessica Jennings-Gaines, Samantha E Allen

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引用次数: 0

Abstract

Chronic wasting disease (CWD) is a fatal transmissible spongiform encephalopathy affecting cervids. Transmission occurs through oral and nasal mucosal exposure to infectious prions (PrPCWD), causing cellular prion proteins (PrPC) to misfold. Currently, the approved diagnostic assays for CWD are immunohistochemistry (IHC) and ELISA. While IHC is considered the gold standard, ELISA is used by most diagnostic laboratories as a screening test for free-ranging cervids. Currently, two tissues have been validated for use with the cervid ELISA test: the medial retropharyngeal lymph node (MRPLN) and the obex region of the brainstem. Palatine tonsil has not been validated for use with the ELISA despite trafficking of PrPCWD to the tonsils early in the course of disease in deer. Here we assessed the suitability of palatine tonsil for CWD testing of free-ranging mule deer (Odocoileus hemionus) using two commercially available ELISA kits in conjunction with IHC. We tested paired MRPLN and tonsillar tissues, collected from hunter-harvested and non-hunter-harvested mule deer (n=176) in Wyoming, US, from 2020 to 2024. Our findings indicated 100% agreement between MRPLN and palatine tonsil, with no difference in the performance of the two commercially available ELISA kits, confirming that palatine tonsil can be substituted for MRPLN for CWD surveillance in free-ranging mule deer with high confidence.

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ELISA法检测散养骡鹿（Odocoileus hemionus）慢性耗损病的评价。

慢性消耗性疾病（CWD）是一种影响宫颈的致命性海绵状脑病。传播通过口腔和鼻腔粘膜暴露于感染性朊病毒（PrPCWD），导致细胞朊病毒蛋白（PrPC）错误折叠。目前，被批准的CWD诊断方法是免疫组织化学（IHC）和ELISA。虽然免疫组化被认为是金标准，但ELISA被大多数诊断实验室用作自由放养提供者的筛查试验。目前，两种组织已被证实可用于宫颈ELISA试验：内侧咽后淋巴结（MRPLN）和脑干的肥胖区域。尽管在鹿的疾病过程早期，PrPCWD被贩运到扁桃体，但Palatine扁桃体尚未被证实与ELISA一起使用。在这里，我们使用两种市售的ELISA试剂盒和免疫组化方法，评估了普氏扁桃体对自由放养的骡鹿（Odocoileus hemionus） CWD检测的适用性。我们测试了配对的MRPLN和扁桃体组织，这些组织是在2020年至2024年期间从美国怀俄明州狩猎和非狩猎的骡鹿（n=176）身上收集的。我们的研究结果表明MRPLN和腭扁桃体之间100%一致，两种市售ELISA试剂盒的性能没有差异，证实了在自由放养的骡鹿中，腭扁桃体可以替代MRPLN进行CWD监测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Wildlife Diseases 农林科学-兽医学

CiteScore

2.70

自引率

0.00%

发文量

213

审稿时长

6-16 weeks

期刊介绍： The JWD publishes reports of wildlife disease investigations, research papers, brief research notes, case and epizootic reports, review articles, and book reviews. The JWD publishes the results of original research and observations dealing with all aspects of infectious, parasitic, toxic, nutritional, physiologic, developmental and neoplastic diseases, environmental contamination, and other factors impinging on the health and survival of free-living or occasionally captive populations of wild animals, including fish, amphibians, reptiles, birds, and mammals. Papers on zoonoses involving wildlife and on chemical immobilization of wild animals are also published. Manuscripts dealing with surveys and case reports may be published in the Journal provided that they contain significant new information or have significance for better understanding health and disease in wild populations. Authors are encouraged to address the wildlife management implications of their studies, where appropriate.