Studying the effect of Chlamydia trachomatis, Helicobacter pylori, and Varicella zoster microorganisms in stimulating the expression of cytokines TNFα, IFNɤ, TGFβ, IL-10 in Alzheimer and non-Alzheimer patients

IF 2.5 4区医学 Q3 NEUROSCIENCES

Neuroscience Letters Pub Date : 2025-03-15 DOI:10.1016/j.neulet.2025.138192

Sima Taheri , Mansoureh Paknejadi , Davoud Esmaeili , Atousa Ferdousi , Mohammad Hassan Shahhosseiny

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引用次数: 0

Abstract

Objectives

This study aimed to use the real-time RT-PCR method to detect the gene expression cytokines IL-10, TNFα, IFN-γ, and TGF-β in the serum of Alzheimer’s patients.

Methods

This study was conducted on 100 serum samples of Alzheimer’s patients. DNA extraction was performed on the samples with the Cinnaclone kit and PCR techniques were used to detect the presence of Helicobacter pylori, Chlamydia trachomatis, and Varicella zoster virus. Real-time RT-PCR was performed to measure the expression of TNFα, IFNɤ, TGFβ, and IL-10 genes with a Smobio kit.

Results

The relative changes in the expression of TNFα, IFNɤ, TGFβ, and IL-10 genes were observed in Alzheimer’s patients compared to the control samples without microorganisms, and a significant increase was observed (P < 0.05).

Conclusion

This study showed that the cytokines TNFα, IFNɤ, TGFβ, and IL-10, have an increase in Alzheimer’s patients(P < 0.05). Therefore, the presence of the microorganisms accompanied by the rise and inducing the expression of cytokines compared to the groups without the mentioned microorganisms causes a significant increase in the production of cytokines effective in the occurrence or exacerbation of Alzheimer’s disease.

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来源期刊

Neuroscience Letters 医学-神经科学

CiteScore

5.20

自引率

0.00%

发文量

408

审稿时长

50 days

期刊介绍： Neuroscience Letters is devoted to the rapid publication of short, high-quality papers of interest to the broad community of neuroscientists. Only papers which will make a significant addition to the literature in the field will be published. Papers in all areas of neuroscience - molecular, cellular, developmental, systems, behavioral and cognitive, as well as computational - will be considered for publication. Submission of laboratory investigations that shed light on disease mechanisms is encouraged. Special Issues, edited by Guest Editors to cover new and rapidly-moving areas, will include invited mini-reviews. Occasional mini-reviews in especially timely areas will be considered for publication, without invitation, outside of Special Issues; these un-solicited mini-reviews can be submitted without invitation but must be of very high quality. Clinical studies will also be published if they provide new information about organization or actions of the nervous system, or provide new insights into the neurobiology of disease. NSL does not publish case reports.