Paenibacillus campi sp. nov., a novel endophytic bacterium isolated from Pennisetum species.

Abstract

Two facultatively aerobic, Gram-stain-positive, rod-shaped, endospore-forming, endophytic bacteria, designated SGZ-1009^T and SGZ-1014, were isolated from the plant Pennisetum sp. Strain SGZ-1009^T grew at 5-50 °C, pH 4.5-11.0 and tolerated up to 4.5% NaCl (w/v), while strain SGZ-1014 exhibited growth at a temperature range of 10-50 °C, pH range 4.5-11.0 and tolerated up to 4.5% NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these two strains belong to the genus Paenibacillus, closely related to the reference type strain of Paenibacillus wenxiniae DSM 100576^T (similarity of 97.0%). The genomic relatedness values for both strains, SGZ-1009^T and SGZ-1014, compared to their closest reference strain P. wenxiniae DSM 100576^T, were 81.6% for average nucleotide identity and 26.0% for digital DNA-DNA hybridization, suggesting strain SGZ-1009^T represents a novel species. The genomic DNA G+C content of strain SGZ-1009^T was 46.4%. Both strains shared anteiso-C_15:0 (55.9%) as the predominant fatty acid, menaquinone-7 as the major respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine as the major polar lipids. Based on these results, strains SGZ-1009^T and SGZ-1014 represent a novel species of Paenibacillus, for which the name Paenibacillus campi sp. nov. is proposed. The type strain is SGZ-1009^T (=MCCC 1K08892^T=GDMCC 1.4280^T=KCTC 43677^T=JCM 36670^T).