Gene expression patterns of the four cardiac chambers in the Thoroughbred horse

IF 1.3 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science Pub Date : 2025-03-09 DOI:10.1016/j.jevs.2025.105415

Charlotte E. Edling , Magdalena Arevalo-Turrubiarte , Antoine Premont , Marcos Castellanos Uribe , Bronte Forbes , Victoria Kemp , Joe Weir , Celia Marr , Rebecca Lewis , Kamalan Jeevaratnam

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Abstract

Athlete horses’ contraction and conduction of the healthy heart influences racing performance. Gene expression patterns in the horse heart are not yet fully investigated. We aim to evaluate the gene expression of the four chambers of the heart overall and with focus on genes involved in the electrophysiology of the heart in Thoroughbred racehorses with no clinical cardiac abnormalities. Tissue was collected from the left atrium (LA), right atrium (RA), left ventricle (LV), and right ventricle (RV). Total RNA was analysed by microarray technique. We compared gene expression in the heart chambers by contrasting atrial against ventricular chambers (chamber related differences), and by contrasting left side to right side (left-to-right related differences).

The pathway analyses revealed that RA was characterised by significantly lower expression of genes related to energy derivation and metabolism in comparison to both ventricles and left atria. LA, on the other hand, was characterised by higher expression of genes related to cardiac conduction, and less expression of cardiac morphogenesis, compared to ventricles. The left-to-right related comparisons indicated wider differences between the atria than between the ventricles. Mapping of the genes specifically involved in cardiac conduction and contraction indicated clear chamber related differences. Some potassium channels, KCNE1 and KCNJ2,3 and 4, showed distinct atrial-ventricular specificity and genes involved in calcium regulation were, as a group, more abundant in both atria compared to ventricles.

Our results provide a general overview of the gene expression pattern in the healthy racehorse, with particular focus on the cardiac ion channels.

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简短交流：“纯种马四个心脏腔的基因表达模式”。

运动员马健康心脏的收缩和传导影响着比赛成绩。马心脏的基因表达模式尚未得到充分研究。我们旨在全面评估心脏四个腔室的基因表达，重点关注无临床心脏异常的纯种赛马的心脏电生理相关基因。分别从左心房（LA）、右心房（RA）、左心室（LV）和右心室（RV）采集组织。用微阵列技术分析总RNA。我们通过对比心房和心室（心室相关差异），以及对比左侧和右侧（左到右相关差异）来比较心室中的基因表达。通路分析显示，与心室和左心房相比，RA的特征是与能量衍生和代谢相关的基因表达明显较低。另一方面，与心室相比，LA的特点是与心脏传导相关的基因表达较高，而心脏形态发生的表达较少。从左到右的相关比较表明心房之间的差异比心室之间的差异更大。特异参与心脏传导和收缩的基因图谱显示出明显的心室相关差异。一些钾离子通道KCNE1和KCNJ2、3和4表现出明显的房室特异性，参与钙调节的基因作为一个群体在心房中比在心室中更丰富。我们的研究结果提供了健康赛马基因表达模式的总体概述，特别关注心脏离子通道。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Equine Veterinary Science 农林科学-兽医学

CiteScore

2.70

自引率

7.70%

发文量

249

审稿时长

77 days

期刊介绍： Journal of Equine Veterinary Science (JEVS) is an international publication designed for the practicing equine veterinarian, equine researcher, and other equine health care specialist. Published monthly, each issue of JEVS includes original research, reviews, case reports, short communications, and clinical techniques from leaders in the equine veterinary field, covering such topics as laminitis, reproduction, infectious disease, parasitology, behavior, podology, internal medicine, surgery and nutrition.