Microtubule Deformation Modulates Intracellular Transport by Kinesin Differently Than Dynein

Abstract

Mechanical stress on cells is transmitted through many biological processes, for example, cell shape control, tissue patterning, and axonal homeostasis. Microtubules, a cytoskeletal component, presumably play a significant role in the mechanoregulation of cellular processes. We investigate motor protein-driven transport of quantum dots along mechanically deformed microtubules. We found that microtubule deformation significantly slowed kinesin-driven transport, whereas we previously reported dynein-driven transport was rather robust. Such dualistic modulation of transportation dynamics of the motor proteins by microtubule deformation can be attributed to the altered affinity of the motor proteins for buckled microtubules. Our results may form the basis for understanding microtubules’ role in regulating cellular processes in a mechanically adverse environment through its detection ability and response to mechanical stress.