m⁶A transferase KIAA1429 mediates the upregulation of LncRNA LINC00968 promoting the progression of gastric cancer cells.

IF 2.7 3区生物学

Hereditas Pub Date : 2025-03-11 DOI:10.1186/s41065-025-00393-9

Huijun Liu, Menghan Yang, Chunyue Zhang, Yanmin Zhang, Yan Wang, Yueda Chen

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引用次数: 0

Abstract

Background: The screening and monitoring of gastric cancer is still a clinical challenge. Both N⁶-methyladenosine (m⁶A) and lncRNAs have been evidenced as critical regulators of gastric cancer, but their interaction and potential in modulating tumor progression remain unclear. This study aimed to evaluate the function of lncRNA LINC00968 in gastric cancer biological processes, and we discovered the role of KIAA1429, a typical m⁶A eraser, in mediating LINC00968 function.

Materials and methods: The expression of LINC00968 was assessed using PCR and regulated by cell transfection. Cellular processes were evaluated by CCK8 and Transwell assays. The m⁶A modification and the interaction of LINC00968 with KIAA1429 were identified with Methylated RNA immunoprecipitation-qPCR. The regulatory effect of LINC00968 on miR-3202 and VIRMA was estimated by luciferase reporter assay.

Results: Significantly increased LINC00968 was observed in gastric cancer cells. Silencing LINC00968 suppressed gastric cancer cell growth and motility. m⁶A-modified sites were predicted in LINC00968 and overexpressing KIAA1429 enhanced the enrichment and stability of LINC00968 in gastric cancer and reversed the knockdown of LINC00968. The overexpression of KIAA1429 could attenuate the inhibitory effect of LINC00968 knockdown on gastric cancer cellular processes. LINC00968 could negatively regulate the expression of miR-3202, which further regulate VIRMA, the coding gene of KIAA1429, in gastric cancer cells.

Conclusions: LINC00968 contributes to the enhanced cell growth and metastasis of gastric cancer, which was mediated by KIAA1429-mediating m⁶A modification and the miR-3202/VIRMA axis.

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m6A转移酶KIAA1429介导LncRNA LINC00968的上调，促进胃癌细胞的进展。

背景：胃癌的筛查和监测仍然是一个临床挑战。n6 -甲基腺苷（m6A）和lncrna已被证明是胃癌的关键调节因子，但它们在调节肿瘤进展中的相互作用和潜力尚不清楚。本研究旨在评估lncRNA LINC00968在胃癌生物学过程中的功能，我们发现了典型的m6A擦除者KIAA1429在介导LINC00968功能中的作用。材料与方法：采用PCR法检测LINC00968的表达，并通过细胞转染进行调控。细胞过程通过CCK8和Transwell检测进行评估。利用甲基化RNA免疫沉淀- qpcr技术鉴定了m6A的修饰以及LINC00968与KIAA1429的相互作用。通过荧光素酶报告基因法估计LINC00968对miR-3202和VIRMA的调控作用。结果：胃癌细胞中LINC00968表达明显升高。沉默LINC00968抑制胃癌细胞生长和运动。在LINC00968中预测了m6a修饰位点，过表达KIAA1429增强了LINC00968在胃癌中的富集和稳定性，逆转了LINC00968的下调。KIAA1429过表达可减弱LINC00968敲低对胃癌细胞过程的抑制作用。LINC00968可以负向调控miR-3202的表达，进而调控KIAA1429的编码基因VIRMA在胃癌细胞中的表达。结论：LINC00968通过kiaa1429介导的m6A修饰和miR-3202/VIRMA轴介导促进胃癌细胞生长和转移。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Hereditas Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.80

自引率

3.70%

发文量

期刊介绍： For almost a century, Hereditas has published original cutting-edge research and reviews. As the Official journal of the Mendelian Society of Lund, the journal welcomes research from across all areas of genetics and genomics. Topics of interest include human and medical genetics, animal and plant genetics, microbial genetics, agriculture and bioinformatics.