Cerebrospinal fluid profiles of targeted metabolomics on neurotransmitters in patients with post-neurosurgical bacterial meningitis.

IF 4.6 2区医学 Q2 IMMUNOLOGY

Frontiers in Cellular and Infection Microbiology Pub Date : 2025-02-25 eCollection Date: 2025-01-01 DOI:10.3389/fcimb.2025.1484144

Zhaojun Mei, Liao Guan, Ziao Xu, Hongwei Cheng, Lei Ye

{"title":"Cerebrospinal fluid profiles of targeted metabolomics on neurotransmitters in patients with post-neurosurgical bacterial meningitis.","authors":"Zhaojun Mei, Liao Guan, Ziao Xu, Hongwei Cheng, Lei Ye","doi":"10.3389/fcimb.2025.1484144","DOIUrl":null,"url":null,"abstract":"Background: Post-neurosurgical bacterial meningitis (PNBM) is a severe complication in patients receiving neurosurgical treatments. Pathogens and neuroinflammation have been reported to influence metabolites in the microenvironment of the central nervous system. However, information about the relationship between neurotransmitter levels and PNBM is still limited. In this study, we aimed to investigate the diagnostic potential of neurotransmitters for PNBM in the patients with stroke.Methods: In this study, a total of 66 stroke patients were recruited. Among them, 40 patients were complicated with PNBM. We profiled cerebrospinal fluid (CSF) levels of neurotransmitter precursors and metabolites using the targeted metabolomics method, which contained 26 precursors and metabolites of neurotransmitters, using ultra-performance liquid chromatography coupled with mass spectrometry (UPLC/MS).Results: We found that 14 biomarkers were downregulated but 3,4-dihydroxyphenylacetic acid (DOPAC) was upregulated in the CSF of PNBM patients. Among the biomarkers, D-glutamine (AUC=1.000), Boc-D-Tyr-OH (AUC=0.9447), L(+)-arginine (AUC=0.9418), and DOPAC (AUC=0.9173) had strong diagnostic efficiency for PNBM. Bioinformatic analysis showed that tyrosine metabolism, butanoate metabolism, histidine metabolism, alanine, aspartate and glutamate metabolism, glycerophospholipid metabolism, arginine and proline metabolism, and tryptophan metabolism might be involved in the pathogenesis of PNBM. After reviewing previous studies, we found a probable diverse pathophysiological alteration between PNBM and community-acquired bacterial meningitis.Conclusions: In summary, we identified downregulated levels of D-glutamine, Boc-D-Tyr-OH, L(+)-arginine, and phenprobamate, and an upregulated level of DOPAC in CSF to have strong diagnostic efficiencies. The results also offered potential targets for the treatment of PNBM.","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1484144"},"PeriodicalIF":4.6000,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11893869/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Cellular and Infection Microbiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3389/fcimb.2025.1484144","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"IMMUNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Post-neurosurgical bacterial meningitis (PNBM) is a severe complication in patients receiving neurosurgical treatments. Pathogens and neuroinflammation have been reported to influence metabolites in the microenvironment of the central nervous system. However, information about the relationship between neurotransmitter levels and PNBM is still limited. In this study, we aimed to investigate the diagnostic potential of neurotransmitters for PNBM in the patients with stroke.

Methods: In this study, a total of 66 stroke patients were recruited. Among them, 40 patients were complicated with PNBM. We profiled cerebrospinal fluid (CSF) levels of neurotransmitter precursors and metabolites using the targeted metabolomics method, which contained 26 precursors and metabolites of neurotransmitters, using ultra-performance liquid chromatography coupled with mass spectrometry (UPLC/MS).

Results: We found that 14 biomarkers were downregulated but 3,4-dihydroxyphenylacetic acid (DOPAC) was upregulated in the CSF of PNBM patients. Among the biomarkers, D-glutamine (AUC=1.000), Boc-D-Tyr-OH (AUC=0.9447), L(+)-arginine (AUC=0.9418), and DOPAC (AUC=0.9173) had strong diagnostic efficiency for PNBM. Bioinformatic analysis showed that tyrosine metabolism, butanoate metabolism, histidine metabolism, alanine, aspartate and glutamate metabolism, glycerophospholipid metabolism, arginine and proline metabolism, and tryptophan metabolism might be involved in the pathogenesis of PNBM. After reviewing previous studies, we found a probable diverse pathophysiological alteration between PNBM and community-acquired bacterial meningitis.

Conclusions: In summary, we identified downregulated levels of D-glutamine, Boc-D-Tyr-OH, L(+)-arginine, and phenprobamate, and an upregulated level of DOPAC in CSF to have strong diagnostic efficiencies. The results also offered potential targets for the treatment of PNBM.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Frontiers in Cellular and Infection Microbiology IMMUNOLOGY-MICROBIOLOGY

CiteScore

7.90

自引率

7.00%

发文量

1817

审稿时长

14 weeks

期刊介绍： Frontiers in Cellular and Infection Microbiology is a leading specialty journal, publishing rigorously peer-reviewed research across all pathogenic microorganisms and their interaction with their hosts. Chief Editor Yousef Abu Kwaik, University of Louisville is supported by an outstanding Editorial Board of international experts. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide. Frontiers in Cellular and Infection Microbiology includes research on bacteria, fungi, parasites, viruses, endosymbionts, prions and all microbial pathogens as well as the microbiota and its effect on health and disease in various hosts. The research approaches include molecular microbiology, cellular microbiology, gene regulation, proteomics, signal transduction, pathogenic evolution, genomics, structural biology, and virulence factors as well as model hosts. Areas of research to counteract infectious agents by the host include the host innate and adaptive immune responses as well as metabolic restrictions to various pathogenic microorganisms, vaccine design and development against various pathogenic microorganisms, and the mechanisms of antibiotic resistance and its countermeasures.