In situ and in silico localization of major satellite DNAs in the genome of the red-eared slider (Trachemys scripta elegans, Emydidae, Testudines).

IF 1.7 4区 生物学 Q4 CELL BIOLOGY
Svetlana A Romanenko, Dmitry Yu Prokopov, Sergey A Marchenko, Maria M Kulak, Arina V Ilina, Natalia A Serdyukova, Svetlana A Galkina, Vladimir A Trifonov
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引用次数: 0

Abstract

Introduction: Satellite DNA is an important component of the eukaryotic genome. Some satellite DNAs plays an important role in various biological processes. The red-eared slider (Trachemys scripta elegans, 2n=50, C=1.43 pg) belongs to the American freshwater turtle family and is recognised as one of the world's most invasive species. In the T. s. elegans chromosome-level genome assembly, which has been recently published, satellite DNAs comprise only 0.1%. From the repetitive repertoire of the T. elegans genome, only ribosomal DNA genes and telomeric repeats have been localised on the species' chromosomes.

Methods: Using publicly available short-read sequencing data, we conducted de novo identification of the most abundant satellite DNAs in T. s. elegans using TAREAN pipeline. We combined bioinformatics (using blastn) and chromosome mapping by fluorescence in situ hybridization to describe the distribution of major tandem repetitive DNAs. The diversity and distribution of satDNA in the assembled genome of T. s. elegans were explored using the SatXplor pipeline.

Results: Six major satellite sequences occupying approximately 0.8% of the genome were identified in the genome data, all of which were successfully localised both in situ and in silico on T. s. elegans chromosomes and in silico on chromosomal scaffolds. We revealed a complex structural organisation of these sequences: monomers may be moderately or highly variable and they may contain regions homologous to retrotransposons. Cytogenetic mapping showed the accumulation of satellite DNAs in the pericentromeric regions of most chromosomes and in the distal regions of the short arms of submetacentric chromosomes. Comparisons between cytogenetic maps and genome assembly data revealed discrepancies in the number and chromosomal locations of the identified satellite DNA clusters.

Conclusion: The red-eared slider genome has a greater proportion of satellite DNA than was previously reported. These satellites demonstrate no specificity for either macrochromosomes or microchromosomes. Differences between in situ and in silico results indicate the challenges of repetitive sequence assembly, as well as discrepancies between chromosome numbering in the current chromosome-level genome assembly and the physical chromosome map.

红耳滑鼠(Trachemys scripta elegans, Emydidae, Testudines)基因组中主要卫星dna的原位和计算机定位。
卫星DNA是真核生物基因组的重要组成部分。一些卫星dna在各种生物过程中起着重要作用。红耳龟(Trachemys scripta elegans, 2n=50, C=1.43 pg)属于美洲淡水龟科,被认为是世界上最具入侵性的物种之一。在最近发表的秀丽隐杆线虫染色体水平的基因组组装中,卫星dna仅占0.1%。从秀丽隐杆线虫基因组的重复序列中,只有核糖体DNA基因和端粒重复序列被定位在该物种的染色体上。方法:利用公开的短读测序数据,利用TAREAN管道对秀丽隐杆线虫中最丰富的卫星dna进行从头鉴定。我们结合生物信息学(利用blastn)和荧光原位杂交的染色体定位来描述主要串联重复dna的分布。利用SatXplor管道对秀丽隐杆线虫组装基因组中satDNA的多样性和分布进行了研究。结果:在基因组数据中鉴定出6个主要的卫星序列,约占基因组的0.8%,所有这些卫星序列都成功地定位在线虫染色体的原位和计算机上,以及染色体支架上的计算机上。我们揭示了这些序列的复杂结构组织:单体可能是中等或高度可变的,它们可能包含与反转录转座子同源的区域。细胞遗传学图谱显示,卫星dna在大多数染色体的中心点周围区域和亚稳心染色体短臂的远端区域积累。细胞遗传学图谱和基因组组装数据之间的比较揭示了所鉴定的卫星DNA簇的数量和染色体位置的差异。结论:红耳滑鼠基因组中卫星DNA的比例比先前报道的要大。这些卫星对大染色体和微染色体都没有特异性。原位和计算机结果之间的差异表明了重复序列组装的挑战,以及当前染色体水平基因组组装中染色体编号与物理染色体图谱之间的差异。
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来源期刊
Cytogenetic and Genome Research
Cytogenetic and Genome Research 生物-细胞生物学
CiteScore
3.10
自引率
5.90%
发文量
25
审稿时长
1 months
期刊介绍: During the last decades, ''Cytogenetic and Genome Research'' has been the leading forum for original reports and reviews in human and animal cytogenetics, including molecular, clinical and comparative cytogenetics. In recent years, most of its papers have centered on genome research, including gene cloning and sequencing, gene mapping, gene regulation and expression, cancer genetics, comparative genetics, gene linkage and related areas. The journal also publishes key papers on chromosome aberrations in somatic, meiotic and malignant cells. Its scope has expanded to include studies on invertebrate and plant cytogenetics and genomics. Also featured are the vast majority of the reports of the International Workshops on Human Chromosome Mapping, the reports of international human and animal chromosome nomenclature committees, and proceedings of the American and European cytogenetic conferences and other events. In addition to regular issues, the journal has been publishing since 2002 a series of topical issues on a broad variety of themes from cytogenetic and genome research.
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