Decreased Expression of Aquaporins as a Feature of Tubular Damage in Lupus Nephritis.

IF 5.1 2区 生物学 Q2 CELL BIOLOGY
Cells Pub Date : 2025-03-05 DOI:10.3390/cells14050380
Melchior Maxime, Van Eycken Marie, Nicaise Charles, Duquesne Thomas, Longueville Léa, Collin Amandine, Decaestecker Christine, Salmon Isabelle, Delporte Christine, Soyfoo Muhammad
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Abstract

Background: Tubulointerstitial hypoxia is a key factor for lupus nephritis progression to end-stage renal disease. Numerous aquaporins (AQPs) are expressed by renal tubules and are essential for their proper functioning. The aim of this study is to characterize the tubular expression of AQP1, AQP2 and AQP3, which could provide a better understanding of tubulointerstitial stress during lupus nephritis. Methods: This retrospective monocentric study was conducted at Erasme-HUB Hospital. We included 37 lupus nephritis samples and 9 healthy samples collected between 2000 and 2020, obtained from the pathology department. Immunohistochemistry was performed to target AQP1, AQP2 and AQP3 and followed by digital analysis. Results: No difference in AQP1, AQP2 and AQP3 staining location was found between healthy and lupus nephritis samples. However, we observed significant differences between these two groups, with a decrease in AQP1 expression in the renal cortex and in AQP3 expression in the cortex and medulla. In the subgroup of proliferative glomerulonephritis (class III/IV), this decrease in AQPs expression was more pronounced, particularly for AQP3. In addition, within this subgroup, we detected lower AQP2 expression in patients with higher interstitial inflammation score and lower AQP3 expression when higher interstitial fibrosis and tubular atrophy were present. Conclusions: We identified significant differences in the expression of aquaporins 1, 2, and 3 in patients with lupus nephritis. These findings strongly suggest that decreased AQP expression could serve as an indicator of tubular injury. Further research is warranted to evaluate AQP1, AQP2, and AQP3 as prognostic markers in both urinary and histological assessments of lupus nephritis.

水通道蛋白表达降低是狼疮肾炎肾小管损伤的一个特征。
背景:小管间质缺氧是狼疮性肾炎发展为终末期肾病的关键因素。许多水通道蛋白(AQPs)在肾小管中表达,对肾小管的正常功能至关重要。本研究的目的是表征AQP1, AQP2和AQP3的小管表达,从而更好地了解狼疮肾炎时的小管间质应激。方法:本回顾性单中心研究在Erasme-HUB医院进行。我们纳入了2000年至2020年间从病理部门收集的37例狼疮性肾炎样本和9例健康样本。对AQP1、AQP2和AQP3进行免疫组化,然后进行数字化分析。结果:AQP1、AQP2、AQP3的染色位置在健康人与狼疮性肾炎标本中均无差异。然而,我们观察到两组之间存在显著差异,肾皮质AQP1表达减少,皮质和髓质的AQP3表达减少。在增殖性肾小球肾炎(III/IV类)亚组中,AQPs表达的下降更为明显,尤其是AQP3。此外,在该亚组中,我们发现间质炎症评分较高的患者AQP2表达较低,而间质纤维化和小管萎缩较高的患者AQP3表达较低。结论:我们发现水通道蛋白1、2和3在狼疮肾炎患者中的表达存在显著差异。这些结果强烈提示AQP表达降低可能是肾小管损伤的一个指标。需要进一步研究AQP1、AQP2和AQP3作为狼疮性肾炎尿液和组织学评估的预后标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Cells
Cells Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
9.90
自引率
5.00%
发文量
3472
审稿时长
16 days
期刊介绍: Cells (ISSN 2073-4409) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to cell biology, molecular biology and biophysics. It publishes reviews, research articles, communications and technical notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. Full experimental and/or methodical details must be provided.
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