Treatment of corticosteroid-induced myopathy through Filgrastim induced endogenous stem cells mobilization in male albino rats.

IF 2.9 4区 生物学 Q1 ANATOMY & MORPHOLOGY
Tarek Hamdy Abd-Elhamid, Nahla Shahat Ismail, Yahia A Amin, Fatma Y Meligy, Ahmed Talat Galal, Hoda Ahmed M Abdel-Ziz, Maha Abd-El Baki Ahmed
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引用次数: 0

Abstract

Introduction: One of well-known exogenous fluorinated glucocorticoid that is used to treat inflammatory and various autoimmune illnesses is dexamethasone. Dexamethasone is known to cause skeletal muscular weakness and when used for an extended period of time, skeletal muscle undergoes atrophy. Granulocyte colony-stimulating factor (G-CSF) is a glycoprotein that helps mobilize stem cells from bone marrow into peripheral circulation. In order to maintain the function of skeletal muscle, these mobilized stem cells multiply and differentiate into mature myocytes. This study was conducted to investigate to what extent administration of filgrastim, human methionyl granulocyte colony-stimulating factor (G-CSF), ameliorates glucocorticoid-induced skeletal muscles damage in adult male albino rats.

Methods: Thirty adult male albino rats were randomly divided into three groups (ten/group), group I (control group, CG): rats received normal diet and orally given normal saline, group II (dexamethasone group, DG): rats were given dexamethasone at a dose of 0.5mg/kg for one month by intraperitoneal injection, group III (filgrastim group, FG): rats were given dexamethasone at dose of 0.5 mg/kg and on day 15, at the beginning of the third week, they were given Filgrastim at a dose of 20 µg/kg till the end of the 4th week by intraperitoneal injection with dexamethasone. Assessment of CK levels, total body weight and motor activity at different time points were done and skeletal muscles specimens were processed for light microscopy, electron microscopy and immunohistochemistry examination.

Results: Administration of dexamethasone (group II) showed variant types of pathological changes such as elevated CK, decrease in body weight, impairment of muscle activity and histologically myofibrillar disarrangement together with cellular infiltration and edema. Filgrastim group showed significant reduction in most of those manifestations. Administration of filgrastim with dexamethasone meliorated most of the symptoms related to dexamethasone induced-myopathy.

Conclusion: Filgrastim administration recovered manifestations of skeletal muscle injuries caused by dexamethasone.

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来源期刊
Cells Tissues Organs
Cells Tissues Organs 生物-发育生物学
CiteScore
4.90
自引率
3.70%
发文量
45
审稿时长
6-12 weeks
期刊介绍: ''Cells Tissues Organs'' aims at bridging the gap between cell biology and developmental biology and the emerging fields of regenerative medicine (stem cell biology, tissue engineering, artificial organs, in vitro systems and transplantation biology). CTO offers a rapid and fair peer-review and exquisite reproduction quality. Special topic issues, entire issues of the journal devoted to a single research topic within the range of interests of the journal, are published at irregular intervals.
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