Malondialdehyde (MDA) and 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels in canine serum: establishing reference intervals and influencing factors.

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES
B Perez-Montero, M L Fermin-Rodriguez, M Portero-Fuentes, J Sarquis, S Caceres, J C Illera Del Portal, L de Juan, G Miro, F Cruz-Lopez
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引用次数: 0

Abstract

Background: Mounting evidence suggests that malondialdehyde (MDA) and 8-hydroxy-2'-deoxyguanosine (8-OHdG) are valuable biomarkers of lipid and nucleic acid oxidation in numerous canine diseases. However, their application in clinical settings is limited due to the absence of reference intervals (RI) and the analytical inconsistencies. Therefore, this study aimed to characterize serum MDA and 8-OHdG concentrations in dogs, to establish assay-specific RI, and to identify biological, haematological and biochemical factors influencing these markers.

Methods: A total of 190 clinically healthy dogs were recruited, including pet dogs, working dogs and shelter dogs. Serum MDA concentration was measured by the Thiobarbituric Acid Reactive Substances (TBARS) assay, while 8-OHdG levels were determined by using a competitive ELISA. RI were established by non-parametric methods. Potential associations between oxidative stress (OS) biomarkers and multiple biological, haematological and biochemical factors were assessed using multivariate regression models.

Results: RI for serum MDA (1.85-14.51 µM) and 8-OHdG (0.06-0.75 ng/mL) were established in the reference population (144 and 143 dogs, respectively). The multivariate regression model for MDA revealed a positive association with total cholesterol concentration, and a negative association with monocyte count. 8-OHdG level was positively associated with urea concentration. Notably, both models also revealed a significant association between MDA and 8-OHdG. Biological factors, including the age and size of the animals, did not exert a significant influence on the results.

Conclusions: This is the first study to establish serum RI for MDA and 8-OHdG in a large and diverse canine population. Additionally, the multivariate regression models identified relevant haematological and biochemical, but not biological factors that should be considered when interpreting the results. These findings could significantly enhance the application of MDA and 8-OHdG as biomarkers in clinical settings, and promote further exploration of their value in canine diseases.

犬血清丙二醛(MDA)和8-羟基-2′-脱氧鸟苷(8-OHdG)水平:建立参考区间及影响因素
背景:越来越多的证据表明,丙二醛(MDA)和8-羟基-2'-脱氧鸟苷(8-OHdG)是许多犬类疾病中有价值的脂质和核酸氧化的生物标志物。然而,由于缺乏参考区间(RI)和分析不一致,它们在临床环境中的应用受到限制。因此,本研究旨在表征犬血清MDA和8-OHdG浓度,建立测定特异性RI,并确定影响这些标志物的生物学、血液学和生化因素。方法:共招募临床健康犬190只,包括宠物犬、工作犬和收容所犬。采用硫代巴比妥酸反应物质(TBARS)法测定血清丙二醛(MDA)浓度,采用竞争性ELISA法测定8-OHdG水平。采用非参数方法建立RI。使用多元回归模型评估氧化应激(OS)生物标志物与多种生物学、血液学和生化因素之间的潜在关联。结果:在参考人群(144只狗和143只狗)中建立了血清MDA(1.85 ~ 14.51µM)和8-OHdG (0.06 ~ 0.75 ng/mL)的RI。多变量回归模型显示MDA与总胆固醇浓度呈正相关,与单核细胞计数负相关。8-OHdG水平与尿素浓度呈正相关。值得注意的是,这两个模型也显示了MDA和8-OHdG之间的显著关联。生物因素,包括动物的年龄和体型,对结果没有显著影响。结论:这是首次在大型犬种群中建立MDA和8-OHdG血清RI的研究。此外,多元回归模型确定了在解释结果时应考虑的相关血液学和生化因素,而不是生物学因素。这些研究结果将显著促进MDA和8-OHdG作为生物标志物在临床中的应用,并进一步探索其在犬疾病中的价值。
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来源期刊
BMC Veterinary Research
BMC Veterinary Research VETERINARY SCIENCES-
CiteScore
4.80
自引率
3.80%
发文量
420
审稿时长
3-6 weeks
期刊介绍: BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.
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