Endophytic bacteria discovered in oil body organelles of the liverworts Marchantia polymorpha and Radula complanata.

IF 2.4 2区 生物学 Q2 PLANT SCIENCES
Blair C Young, Barbara Thiers, James F White, Lena Struwe
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引用次数: 0

Abstract

Premise: Interactions between endophytic microbes and bryophytes have been understudied. The liverwort oil body has also remained poorly understood since its discovery, and modern studies have failed to ascertain its function and composition. Many liverwort species possess oil bodies with conspicuous granules of unknown structure. We surveyed these granular liverwort oil bodies for the presence of bacteria to improve upon the understanding of liverworts, their oil bodies, and bacterial endophytes in nonvascular land plants.

Methods: Wild-collected specimens from living samples of Marchantia polymorpha and Radula complanata were stained with SYTO-13 and RADA to determine the presence or absence of bacteria within their oil bodies. Samples stained with calcofluor white, SYTO-13, and RADA were observed with confocal fluorescent microscopy for presence of nucleic acids and bacterial peptidoglycan cell walls within oil bodies.

Results: We discovered large masses of bacteria within the oil bodies of M. polymorpha and R. complanata based on the presence of stained nucleic acids and peptidoglycans localized to the oil body "granules". Such bacteria were present in all oil bodies of the two species.

Conclusions: These newly discovered intraorganellar bacteria correspond to the previously described "granules" of oil bodies. The existence of granular oil bodies in many liverwort species implies that this endophytic association may not be isolated to species investigated here. Assessments of additional liverwort species for presence and identity of oil body bacteria are needed to understand this intriguing association in one of the oldest land plant lineages.

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来源期刊
American Journal of Botany
American Journal of Botany 生物-植物科学
CiteScore
4.90
自引率
6.70%
发文量
171
审稿时长
3 months
期刊介绍: The American Journal of Botany (AJB), the flagship journal of the Botanical Society of America (BSA), publishes peer-reviewed, innovative, significant research of interest to a wide audience of plant scientists in all areas of plant biology (structure, function, development, diversity, genetics, evolution, systematics), all levels of organization (molecular to ecosystem), and all plant groups and allied organisms (cyanobacteria, algae, fungi, and lichens). AJB requires authors to frame their research questions and discuss their results in terms of major questions of plant biology. In general, papers that are too narrowly focused, purely descriptive, natural history, broad surveys, or that contain only preliminary data will not be considered.
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