Endophytic bacteria discovered in oil body organelles of the liverworts Marchantia polymorpha and Radula complanata

IF 2.4 2区 生物学 Q2 PLANT SCIENCES
Blair C. Young, Barbara Thiers, James F. White, Lena Struwe
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引用次数: 0

Abstract

Premise

Interactions between endophytic microbes and bryophytes have been understudied. The liverwort oil body has also remained poorly understood since its discovery, and modern studies have failed to ascertain its function and composition. Many liverwort species possess oil bodies with conspicuous granules of unknown structure. We surveyed these granular liverwort oil bodies for the presence of bacteria to improve upon the understanding of liverworts, their oil bodies, and bacterial endophytes in nonvascular land plants.

Methods

Wild-collected specimens from living samples of Marchantia polymorpha and Radula complanata were stained with SYTO-13 and RADA to determine the presence or absence of bacteria within their oil bodies. Samples stained with calcofluor white, SYTO-13, and RADA were observed with confocal fluorescent microscopy for presence of nucleic acids and bacterial peptidoglycan cell walls within oil bodies.

Results

We discovered large masses of bacteria within the oil bodies of M. polymorpha and R. complanata based on the presence of stained nucleic acids and peptidoglycans localized to the oil body “granules”. Such bacteria were present in all oil bodies of the two species.

Conclusions

These newly discovered intraorganellar bacteria correspond to the previously described “granules” of oil bodies. The existence of granular oil bodies in many liverwort species implies that this endophytic association may not be isolated to species investigated here. Assessments of additional liverwort species for presence and identity of oil body bacteria are needed to understand this intriguing association in one of the oldest land plant lineages.

Abstract Image

在多形地茅和扁地瓜油体细胞器中发现的内生细菌。
前提:内生微生物与苔藓植物之间的相互作用已被充分研究。自发现以来,对苔油体的了解也很少,现代研究未能确定其功能和成分。许多苔类植物的油体具有结构不明的明显颗粒。我们调查了这些颗粒状的肝苔油体中细菌的存在,以提高对无维管陆地植物中肝苔、其油体和细菌内生菌的认识。方法:采用SYTO-13染色法和RADA染色法检测野生多形地麻和平拉陀油体中是否存在细菌。在共聚焦荧光显微镜下,用钙荧光白、SYTO-13和RADA染色的样品观察油体内核酸和细菌肽聚糖细胞壁的存在。结果:通过检测到油体“颗粒”上的染色核酸和肽聚糖,我们在多形霉和扁豆油体中发现了大量细菌。这类细菌存在于这两个物种的所有油体中。结论:这些新发现的胞内细菌与先前描述的油体“颗粒”相对应。在许多种苔类植物中存在颗粒状油体,这意味着这种内生关联可能并不局限于本文所研究的物种。为了了解最古老的陆地植物谱系之一中这种有趣的联系,需要对其他苔类进行油体细菌的存在和鉴定。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
American Journal of Botany
American Journal of Botany 生物-植物科学
CiteScore
4.90
自引率
6.70%
发文量
171
审稿时长
3 months
期刊介绍: The American Journal of Botany (AJB), the flagship journal of the Botanical Society of America (BSA), publishes peer-reviewed, innovative, significant research of interest to a wide audience of plant scientists in all areas of plant biology (structure, function, development, diversity, genetics, evolution, systematics), all levels of organization (molecular to ecosystem), and all plant groups and allied organisms (cyanobacteria, algae, fungi, and lichens). AJB requires authors to frame their research questions and discuss their results in terms of major questions of plant biology. In general, papers that are too narrowly focused, purely descriptive, natural history, broad surveys, or that contain only preliminary data will not be considered.
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