Mechanical forces and enzymatic digestion act together to induce the remodeling of collagen fibrils in tumor microenvironment†

IF 6.1 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS
Lab on a Chip Pub Date : 2025-03-07 DOI:10.1039/D4LC00821A
Jiling Shi, Aihua Jing, Qinan Yin, Xuewei Zheng, Zhigang Hu, Xibin Jiao, Yaomin Fan, Xiangyang Zu, Jinghua Li, Yanping Liu, Jiayu Zhai, Xiucheng Li and Kena Song
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引用次数: 0

Abstract

Cancer is a serious disease in human beings, and its high lethality is mainly due to the invasion and metastasis of cancer cells. Clinically, the accumulation and high orientation of collagen fibrils were observed in cancerous tissue, which occurred not only at the location of invasion but also at 10–20 cm from the tumor. Studies indicated that the invasion of cancer cells could be guided by the oriented collagen fibrils, even in a dense matrix characterized by difficulty degradation. So, the orientation of collagen fibrils is closely related to invasion by cancer cells. However, the formation of the orientation of collagen fibrils remains insufficiently studied. A microfluidic chip-based collagen fibril tissue model was established to demonstrate its underlying mechanism. In this article, the dynamic mechanism of collagen fibril reconstruction from free orientation to high orientation was investigated at the mesoscopic dynamic level. In the experiment, the mechanical forces from interstitial flow and cell deformation were confirmed as significant factors for collagen fibril remodeling. Additionally, enzymes were confirmed as an another inducer to reconstruct the morphology of collagen fibrils, the mechanism of which was chemical degradation and recombination. Interstitial flow combined with an enzyme is an excellent combination for remodeling the distal collagen fibrils of a tumor, and this phenomenon was caught in a microfluidic platform with a micro-dose. This study to some extent answers the question of the kinetic mechanism of collagen fibril remodeling, and is expected to provide support for further proposed strategies to inhibit the orientation reconstruction of collagen fibrils and cancer treatment and prognosis.

Abstract Image

机械力和酶促消化共同作用,诱导肿瘤微环境中胶原原纤维的重塑。
癌症是人类的一种严重疾病,其高致死率主要是由于癌细胞的侵袭和转移。临床观察到癌组织中胶原原纤维的堆积和高取向,不仅发生在侵袭部位,而且发生在距肿瘤10-20 cm处。研究表明,即使在难以降解的致密基质中,定向的胶原原纤维也可以引导癌细胞的侵袭。因此,胶原原纤维的取向与癌细胞的侵袭密切相关。然而,胶原原纤维取向的形成仍未得到充分的研究。建立了基于微流控芯片的胶原原纤维组织模型,以验证其潜在机制。本文从介观动力学水平研究了胶原原纤维从自由取向向高取向重建的动力学机制。在实验中,证实了间质流动和细胞变形的机械力是胶原纤维重塑的重要因素。此外,酶被证实是重建胶原原纤维形态的另一种诱导剂,其机制是化学降解和重组。间质流动与酶结合是肿瘤远端胶原原纤维重塑的绝佳组合,这种现象在微剂量的微流控平台上被捕捉到。本研究在一定程度上回答了胶原原纤维重塑的动力学机制问题,并有望为进一步提出抑制胶原原纤维定向重建的策略以及癌症的治疗和预后提供支持。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Lab on a Chip
Lab on a Chip 工程技术-化学综合
CiteScore
11.10
自引率
8.20%
发文量
434
审稿时长
2.6 months
期刊介绍: Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.
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