Altered diversity and composition of gut microbiota in Korean children with food allergy

IF 4.6 2区医学 Q2 ALLERGY

Clinical and Translational Allergy Pub Date : 2025-03-12 DOI:10.1002/clt2.70036

Minyoung Jung, Ji Young Lee, Sukyung Kim, Jeongmin Song, Sehun Jang, Sanghee Shin, Min Hee Lee, Mi Jin Kim, Jiwon Kim, Han Byul Lee, Yeonghee Kim, Kangmo Ahn, Minji Kim, Jihyun Kim

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引用次数: 0

Abstract

Background

This study aimed to comprehensively characterize the gut microbiome and identify individual and grouped gut microbes associated with food allergy (FA) using 16S rRNA gene sequencing.

Methods

Fecal samples were collected from children with IgE-mediated FA and from sex- and age-matched controls. The V3–V4 variable regions of the 16S rRNA gene of the gut microbiome were profiled using next-generation sequencing (Illumina, USA). Bacterial species richness, intracommunity diversity, and intergroup dissimilarity were evaluated. Functional profiles were predicted using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) and the Minimal Set of Pathways (MinPath) algorithm.

Results

Fecal samples were collected from children with IgE-mediated FA (n = 66) and from sex- and age-matched controls (n = 22). Gut microbiome richness (p < 0.0001), intra-community diversity (p < 0.0001), and inter-community diversity (p = 0.0004) were higher in the healthy group than in the FA group. Patients with FA were enriched in Blautia, Fusicatenibacter, and Ruminococcus_g5 compared with healthy control individuals (all p < 0.05). Healthy control individuals were significantly enriched in Oscillibacter and Ruminococcus compared with patients with FA (all p < 0.05). Functional pathway analysis identified enrichment in pathways related to endoglucanase in healthy controls and the ATP-binding cassette (ABC) transport system in FA patients.

Conclusions

The gut microbiomes of patients with FA and healthy control individuals had different taxonomic abundances, and the microbiome richness and diversity of the bacterial flora of patients with FA were reduced compared with controls.

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韩国食物过敏儿童肠道菌群多样性和组成的改变

本研究旨在通过16S rRNA基因测序，全面表征肠道微生物组，鉴定与食物过敏（FA）相关的个体和肠道微生物群。方法收集ige介导的FA患儿和性别、年龄相匹配的对照组的粪便样本。使用下一代测序（Illumina， USA）对肠道微生物组16S rRNA基因的V3-V4可变区进行了分析。对细菌种类丰富度、群落内多样性和群间差异性进行了评价。利用重构未观察状态（PICRUSt）和最小路径集（MinPath）算法对群落的系统发育调查进行了功能预测。结果收集了ige介导的FA患儿（n = 66）和性别和年龄匹配的对照组（n = 22）的粪便样本。肠道菌群丰富度(p <；0.0001)，群落内多样性(p <；0.0001)，健康组的群落间多样性（p = 0.0004）高于FA组。与健康对照组相比，FA患者中Blautia、Fusicatenibacter和Ruminococcus_g5含量丰富(p <；0.05)。与FA患者相比，健康对照个体的Oscillibacter和Ruminococcus显著富集(p <；0.05)。功能通路分析发现，健康对照中与内切葡聚糖酶相关的通路富集，FA患者中与atp结合盒（ABC）运输系统相关的通路富集。结论FA患者与健康对照者肠道菌群的分类丰度存在差异，FA患者肠道菌群的丰富度和多样性均低于对照组。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical and Translational Allergy Immunology and Microbiology-Immunology

CiteScore

7.50

自引率

4.50%

发文量

117

审稿时长

12 weeks

期刊介绍： Clinical and Translational Allergy, one of several journals in the portfolio of the European Academy of Allergy and Clinical Immunology, provides a platform for the dissemination of allergy research and reviews, as well as EAACI position papers, task force reports and guidelines, amongst an international scientific audience. Clinical and Translational Allergy accepts clinical and translational research in the following areas and other related topics: asthma, rhinitis, rhinosinusitis, drug hypersensitivity, allergic conjunctivitis, allergic skin diseases, atopic eczema, urticaria, angioedema, venom hypersensitivity, anaphylaxis, food allergy, immunotherapy, immune modulators and biologics, animal models of allergic disease, immune mechanisms, or any other topic related to allergic disease.