Protective Effects and Mechanisms of Active Fractions of Ixeris sonchifolia on Rats With Toxic Heat and Blood Stasis Syndrome Revealed by Pharmacodynamics and Metabonomics

IF 1.8 4区 医学 Q4 BIOCHEMICAL RESEARCH METHODS
Zhaowei Liu, Yuanyuan Zhao, Yiran Ren, Yifei Liu, Zhenqing Liu, Jingxuan Zhang, Ying Liu
{"title":"Protective Effects and Mechanisms of Active Fractions of Ixeris sonchifolia on Rats With Toxic Heat and Blood Stasis Syndrome Revealed by Pharmacodynamics and Metabonomics","authors":"Zhaowei Liu,&nbsp;Yuanyuan Zhao,&nbsp;Yiran Ren,&nbsp;Yifei Liu,&nbsp;Zhenqing Liu,&nbsp;Jingxuan Zhang,&nbsp;Ying Liu","doi":"10.1002/bmc.70042","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p><i>Ixeris sonchifolia</i> (IS) has been demonstrated to have beneficial effects on clearing heat and detoxifying, promoting blood circulation and removing blood stasis. However, the protective effects of active fractions and the underlying mechanisms of IS against toxic heat and blood stasis syndrome (THBSS) remain unclear. This study aimed to investigate this. In this study, the protective effects of 30%, 50%, and 70% ethanol fractions of IS on rats with THBSS were evaluated by observing myocardial pathology, analyzing blood rheology and blood routine indices, and the general conditions of the rats. Comprehensive metabolomic analysis of plasma and myocardium samples was conducted using ultra-high-performance liquid chromatography linear ion trap quadrupole orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap MS/MS). The results showed that IS-30% had a significant protective effect on rats with THBSS, and it could play a protective role by modulating 35 differential metabolites associated with key metabolic pathways, including glycerophospholipid metabolism, sphingosine metabolism, alpha-linolenic acid metabolism, and nicotinate and nicotinamide metabolism. Analysis of different metabolites and pathways showed that they are closely associated with inflammatory responses; therefore, it is hypothesized that IS-30% may protect against THBSS by exerting an anti-inflammatory effect. This study provides valuable data supporting the druggability of IS-30% and offers new insights into its potential therapeutic applications.</p>\n </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 4","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical Chromatography","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/bmc.70042","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0

Abstract

Ixeris sonchifolia (IS) has been demonstrated to have beneficial effects on clearing heat and detoxifying, promoting blood circulation and removing blood stasis. However, the protective effects of active fractions and the underlying mechanisms of IS against toxic heat and blood stasis syndrome (THBSS) remain unclear. This study aimed to investigate this. In this study, the protective effects of 30%, 50%, and 70% ethanol fractions of IS on rats with THBSS were evaluated by observing myocardial pathology, analyzing blood rheology and blood routine indices, and the general conditions of the rats. Comprehensive metabolomic analysis of plasma and myocardium samples was conducted using ultra-high-performance liquid chromatography linear ion trap quadrupole orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap MS/MS). The results showed that IS-30% had a significant protective effect on rats with THBSS, and it could play a protective role by modulating 35 differential metabolites associated with key metabolic pathways, including glycerophospholipid metabolism, sphingosine metabolism, alpha-linolenic acid metabolism, and nicotinate and nicotinamide metabolism. Analysis of different metabolites and pathways showed that they are closely associated with inflammatory responses; therefore, it is hypothesized that IS-30% may protect against THBSS by exerting an anti-inflammatory effect. This study provides valuable data supporting the druggability of IS-30% and offers new insights into its potential therapeutic applications.

从药效学和代谢组学的角度探讨红叶黄有效部位对中热血瘀证大鼠的保护作用及其机制
已被证明具有清热解毒、活血化瘀的有益作用。然而,黄芪活性组分对中热血瘀证(THBSS)的保护作用及其机制尚不清楚。本研究旨在对此进行调查。本研究通过观察心肌病理、分析血液流变学和血常规指标以及大鼠一般情况,评价30%、50%、70%乙醇组分IS对THBSS大鼠的保护作用。采用超高效液相色谱-线性离子阱-四极轨道阱质谱(UHPLC-LTQ-Orbitrap MS/MS)对血浆和心肌样品进行综合代谢组学分析。结果表明,IS-30%对THBSS大鼠具有显著的保护作用,其可能通过调节甘油磷脂代谢、鞘氨醇代谢、α -亚麻酸代谢、烟酸和烟酰胺代谢等35种关键代谢途径相关的差异代谢物发挥保护作用。对不同代谢产物和途径的分析表明,它们与炎症反应密切相关;因此,我们假设is -30%可能通过发挥抗炎作用来预防THBSS。本研究提供了有价值的数据,支持IS-30%的可药物性,并为其潜在的治疗应用提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Biomedical Chromatography
Biomedical Chromatography 生物-分析化学
CiteScore
3.60
自引率
5.60%
发文量
268
审稿时长
2.3 months
期刊介绍: Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信