Molecular insights from 2023 and 2024 outbreaks reveal exclusive circulation of peste des petits ruminants virus lineage IV in India

IF 2.5 4区医学 Q3 VIROLOGY

Archives of Virology Pub Date : 2025-03-13 DOI:10.1007/s00705-025-06254-0

Asha Anand, Swathi Mahadevappa, Rakshit Ojha, Archana Pal, Shweta Priya, Annett Helcita Dsouza, Prajakta Prashant Bokade, Kirubakaran Vinod Kumar, Divakar Hemadri, Baldev Raj Gulati, Vinayagamurthy Balamurugan

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Abstract

Peste des petits ruminants (PPR) is a highly contagious disease affecting sheep and goats, caused by PPR virus (PPRV), a member of the genus Morbillivirus. In 2023 and 2024, on testing of suspected clinical samples for PPR received through passive surveillance of sheep and goat flocks in various states of India resulted in the documentation of 15 laboratory-confirmed outbreaks of PPR. During the outbreak investigation, morbidity and significant mortality rates were observed, and clinical samples collected from the affected flocks were tested using ELISA and RT-PCR, confirming the presence of PPRV. We studied in detail nine out of 15 confirmed outbreaks for characterization of virus including partial sequencing of nucleocapsid (N) and fusion (F) genes of the PPRV. In addition, the virus from each outbreak was isolated from tissue and swab samples by blind passage in Vero cells. Phylogenetic analysis based on partial N gene sequences revealed that all of the Indian isolates belonged to Asian lineage IV. This study confirms that all of the cases of PPR reported so far in India have been caused by PPRV lineage IV.

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.