A sequential drug delivery system based on silk fibroin scaffold for effective cartilage repair

IF 18 1区医学 Q1 ENGINEERING, BIOMEDICAL

Bioactive Materials Pub Date : 2025-03-12 DOI:10.1016/j.bioactmat.2025.03.005

Menglin Xiao , Liangyan Sun , Kang Wu , Yuqi Ding , Peipei Wang , Chuangchuang Mu , Jinrong Yao , Zhengzhong Shao , Bingjiao Zhao , Xin Chen

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引用次数: 0

Abstract

Endogenous repair of cartilage defects is a preferential strategy for cartilage repair, but always hindered by insufficient early-stage cells and incomplete cell differentiation at later stages. For in-situ cartilage regeneration, it is crucial to develop a sequential drug release system capable of recruiting endogenous bone marrow mesenchymal stem cells (BMSCs) and promoting their chondrogenic differentiation. Herein, based on our long-term and fruitful research on silk fibroin (SF) porous scaffolds, a cell-free sequential drug delivery SF scaffold was developed. BMSCs affinity peptide PFSSTKT (PFS) was coated on the surface of SF scaffold, in which chondrogenic inducer kartogenin (KGN) and anti-inflammatory factor dexamethasone (DEX) were loaded. PFS was rapidly released within the first 10 days while KGN and DEX could be released over 28 days. The scaffold promoted BMSCs migration and chondrogenic differentiation through the release of PFS and KGN in vitro. Finally, the sequential drug released by the implanted SF scaffolds in rats indeed recruited endogenous BMSCs and significantly promoted the in-situ regeneration of their knee cartilage defects. In summary, this study not only introduces a green and environmentally friendly all silk-based sequential drug delivery system, but also provides an effective tissue engineering functional scaffold for in-situ cartilage regeneration.

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来源期刊

Bioactive Materials Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

28.00

自引率

6.30%

发文量

436

审稿时长

20 days

期刊介绍： Bioactive Materials is a peer-reviewed research publication that focuses on advancements in bioactive materials. The journal accepts research papers, reviews, and rapid communications in the field of next-generation biomaterials that interact with cells, tissues, and organs in various living organisms. The primary goal of Bioactive Materials is to promote the science and engineering of biomaterials that exhibit adaptiveness to the biological environment. These materials are specifically designed to stimulate or direct appropriate cell and tissue responses or regulate interactions with microorganisms. The journal covers a wide range of bioactive materials, including those that are engineered or designed in terms of their physical form (e.g. particulate, fiber), topology (e.g. porosity, surface roughness), or dimensions (ranging from macro to nano-scales). Contributions are sought from the following categories of bioactive materials: Bioactive metals and alloys Bioactive inorganics: ceramics, glasses, and carbon-based materials Bioactive polymers and gels Bioactive materials derived from natural sources Bioactive composites These materials find applications in human and veterinary medicine, such as implants, tissue engineering scaffolds, cell/drug/gene carriers, as well as imaging and sensing devices.