Maximizing harvesting of Chlorella vulgaris via calcium oxide nanoparticle-modified fungal pellet using response surface methodology

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal Pub Date : 2025-03-01 DOI:10.1016/j.bej.2025.109700

Muhammad Hizbullahi Usman , Mohd Farizal Kamaroddin , Mohd Helmi Sani , Aliyu Ibrahim Dabai , Abdulrahman Sani Aliero , Ali El-Rayyes

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引用次数: 0

Abstract

This study aims to optimize the harvesting of Chlorella vulgaris using calcium oxide nanoparticle modified-Aspergillus pseudonomiae pellets (AP@CaONP). To maximize harvesting efficiency, response surface methodology (RSM-CCD) was applied to statistically optimize key parameters, including mycelial dosage (%), agitation speed (rpm), and pH. Lipid yield from harvested C. vulgaris biomass under optimized conditions was analyzed, and resulting fatty acid methyl esters were identified using Gas Chromatography-Mass Spectrometry (GC-MS). Additionally, the recycling potential of spent medium for recultivation of C. vulgaris was evaluated. Scanning Field Emission Scanning Electron Microscopy (FESEM), Fourier Transform Infrared spectroscopy (FTIR), and zeta potential analyses were conducted to confirm the interaction between AP@CaONP and C. vulgaris. The results show that under optimized conditions (6 % mycelial dosage, 170 rpm, pH 5.5), harvesting efficiency reached 99.24 % in 60 minutes with only a 0.2 % deviation between predicted and experimental values. Lipid yield from harvested biomass reached 26.98 %, and GC-MS revealed fatty acids including C16:0, C16:1, C16:2, C18:0, C18:1, and C18:2. C. vulgaris showed similar growth patterns in fresh and recycled BG-11 media, indicating minimal impact on biomass productivity and reduced water consumption. This optimized AP@CaONP-based harvesting method offers a practical, eco-friendly approach for efficient microalgae biomass recovery, supporting sustainable biodiesel production from Chlorella species.

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来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.