Characterizing a new rat model of chronic pain after spine surgery

IF 14.3 1区 医学 Q1 CELL & TISSUE ENGINEERING
Qichao Wu, Neil C. Ford, Shaoqiu He, Chi Zhang, Xiang Cui, Jing Liu, Xueming Chen, Xu Cao, Yun Guan, Lei Zang
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Abstract

Chronic pain after spine surgery (CPSS) is a complex disorder characterized by multifactorial pathogenesis that occurs in 8%–40% of patients undergoing lumbar spine surgery. We aimed to develop a rat model that mimics clinical CPSS conditions by taking two sequential surgical procedures. Step 1: A plastic rod was inserted into the left L5 intervertebral foramen to produce a steady compression on the dorsal root ganglion (DRG) and the spinal nerve, a common cause of low back pain (LBP). Step 2: The rod was removed after 7 days when rats exhibited mechanical and heat hypersensitivity in the ipsilateral hindpaw, followed by a full L5 laminectomy to mimic spine decompression surgery in LBP patients. The retention of the rod induced a prolonged LBP-like behavior but was quickly resolved after rod removal without laminectomy. However, rats that received laminectomy after rod removal developed heightened mechanical and heat sensitivity in the hindpaw, impaired gait, and reduced spontaneous exploration activity, indicating CPSS. Patch clamp recording revealed a significant augmentation in the intrinsic excitability of small-diameter DRG neurons in CPSS rats. Administration of Dermorphin [D-Arg2, Lys4] (1–4) amide (DALDA, 5 mg /kg, i.p.), a peripherally acting mu-opioid receptor (MOR)-preferred agonist, attenuated pain hypersensitivity, capsaicin-induced [Ca2+]i rising and the increased intrinsic excitability of DRG neurons from CPSS rats. Our findings suggest that this new model, which mirrors the nature of CPSS developed in patients, may be useful for future studies of the underlying mechanisms.

Abstract Image

新型脊柱手术后慢性疼痛大鼠模型的特征描述
脊柱手术后慢性疼痛(CPSS)是一种复杂的疾病,以多因素发病为特征,发生在8%-40%的腰椎手术患者中。我们的目标是通过两次连续的外科手术来建立一个模拟临床CPSS条件的大鼠模型。第一步:将塑料棒插入左L5椎间孔,对背根神经节(DRG)和脊神经产生稳定的压迫,这是腰痛(LBP)的常见原因。步骤2:当大鼠在同侧后爪表现出机械和热超敏反应时,在7天后取出棒,然后进行完整的L5椎板切除术,以模拟LBP患者的脊柱减压手术。棒的保留诱导了长时间的lbp样行为,但在不切除椎板的情况下去除棒后迅速解决。然而,在去除棒后接受椎板切除术的大鼠后爪的机械和热敏性增强,步态受损,自发探索活动减少,表明CPSS。膜片钳记录显示CPSS大鼠小直径DRG神经元的固有兴奋性显著增强。外周作用的多阿片受体(MOR)首选激动剂Dermorphin [D-Arg2, Lys4] (1-4) amide (DALDA, 5 mg /kg, i.p),可减轻CPSS大鼠的疼痛超敏反应,辣椒素诱导的[Ca2+]i升高和DRG神经元的内在兴奋性增加。我们的研究结果表明,这一新模型反映了患者发生的CPSS的本质,可能对未来潜在机制的研究有用。
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来源期刊
Bone Research
Bone Research CELL & TISSUE ENGINEERING-
CiteScore
20.00
自引率
4.70%
发文量
289
审稿时长
20 weeks
期刊介绍: Established in 2013, Bone Research is a newly-founded English-language periodical that centers on the basic and clinical facets of bone biology, pathophysiology, and regeneration. It is dedicated to championing key findings emerging from both basic investigations and clinical research concerning bone-related topics. The journal's objective is to globally disseminate research in bone-related physiology, pathology, diseases, and treatment, contributing to the advancement of knowledge in this field.
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