Diverse Acinetobacter species and Plasmid-Driven spread of carbapenem resistance in pharmaceutical settings in China

IF 9.7 1区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environment International Pub Date : 2025-03-11 DOI:10.1016/j.envint.2025.109373

Jintao He , Liang Hong , Meijun Song , Yisha Zhang , Wang Zhang , Linghong Zhang , Danyan Zhou , Zhouwei Chen , Yunsong Yu , Huan Chen , Xiaoting Hua

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引用次数: 0

Abstract

Acinetobacter species have emerged as a significant public health concern due to their remarkable capacity to acquire antimicrobial resistance. Environmental reservoirs play a crucial role in spreading antimicrobial resistance genes and potentially pathogenic bacteria to clinical settings. However, most studies on nonhuman isolates have focused on a limited sample size. Comprehensive population sampling on One Health principles is essential to monitor the antibiotic resistome and virulome in Acinetobacter spp. Here, we identified three carbapenems-resistant Acinetobacter spp. isolates harboring bla_NDM-1 and discovered two novel Acinetobacter species in pharmaceutical production environments. A total of 94 Acinetobacter spp. strains were isolated from pharmaceutical production environments across 17 cities in China, forming 17 distinct Acinetobacter clusters comprising two novel species and 15 previously known species. Phylogenetic analysis indicated that Acinetobacter spp. isolated from pharmaceutical settings are predominantly confined to these settings. Genomic analysis revealed 10 specific families of bla_CHDL genes in 51 isolates and bla_NDM-1 in three isolates. The overall rates of phenotypic resistance to antimicrobials were low among Acinetobacter spp. isolates, with less than 10 % resistance observed for all tested drugs, and only three isolates carrying bla_NDM-1 were resistant to carbapenems. The bla_NDM-1 gene was located in approximately 49 kb PTU-Pse8 conjugative plasmids with conserved backbones, although plasmid pXH1688-NDM displayed enhanced growth and stability. Two novel Acinetobacter species, A. yuyunsongii sp. nov. and A. chenhuanii sp. nov., were characterized using phenotypic and genomic analyses. Particularly, A. yuyunsongii sp. nov. XH1639 harbors a bla_OXA-58-carrying conjugative plasmid and exhibits multidrug-resistant phenotype. Our study advances Acinetobacter taxonomy and underscores the urgency of monitoring the dynamics of Acinetobacter species in environmental sources to implement effective measures to mitigate transmission risks to healthcare facilities.

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中国制药环境中碳青霉烯耐药的不动杆菌种类和质粒驱动传播

由于不动杆菌具有获得抗菌素耐药性的显著能力，它们已成为一个重大的公共卫生问题。环境宿主在将抗微生物药物耐药性基因和潜在致病菌传播到临床环境中发挥着至关重要的作用。然而，大多数对非人类分离株的研究都集中在有限的样本量上。根据“一个健康”原则进行全面的种群抽样对监测不动杆菌的抗生素抗性组和病毒组至关重要。本研究鉴定了三株含blaNDM-1的耐碳青霉烯不动杆菌，并在制药生产环境中发现了两种新的不动杆菌。从中国17个城市的制药生产环境中分离到94株不动杆菌，形成17个不同的不动杆菌群，包括2个新种和15个已知种。系统发育分析表明，从制药环境中分离的不动杆菌主要局限于这些环境。基因组分析在51株分离株中发现10个blaCHDL基因家族，在3株分离株中发现blaNDM-1基因家族。不动杆菌菌株对抗菌素的总体表型耐药率较低，对所有测试药物的耐药率均低于10% %，只有3株携带blaNDM-1的菌株对碳青霉烯类耐药。blaNDM-1基因位于大约49 kb的PTU-Pse8共轭质粒中，具有保守的骨架，尽管质粒pXH1688-NDM表现出增强的生长和稳定性。利用表型和基因组分析对云南云南两种新型不动杆菌A. yuyunsongii sp.和A. chenhuanii sp.进行了鉴定。特别是，a . yuyunsongii sp. 11 . XH1639携带blaoxa -58结合质粒，并表现出多药耐药表型。我们的研究促进了不动杆菌的分类，并强调了监测环境源中不动杆菌物种动态的紧迫性，以实施有效措施来减轻医疗机构的传播风险。

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来源期刊

Environment International 环境科学-环境科学

CiteScore

21.90

自引率

3.40%

发文量

734

审稿时长

2.8 months

期刊介绍： Environmental Health publishes manuscripts focusing on critical aspects of environmental and occupational medicine, including studies in toxicology and epidemiology, to illuminate the human health implications of exposure to environmental hazards. The journal adopts an open-access model and practices open peer review. It caters to scientists and practitioners across all environmental science domains, directly or indirectly impacting human health and well-being. With a commitment to enhancing the prevention of environmentally-related health risks, Environmental Health serves as a public health journal for the community and scientists engaged in matters of public health significance concerning the environment.