Impact of mild traumatic brain injury (mTBI) on sperm genome integrity: insights from a mouse model.

IF 5.4 2区医学 Q1 Medicine

Journal of Endocrinological Investigation Pub Date : 2025-03-10 DOI:10.1007/s40618-025-02549-w

M Memis, S Taheri, Z Y Sukranlı, E M Duman, B Er, Z Hamurcu, Ahsen Güler, M Rassoulzadegan, Z Karaca, F Tanriverdi, K Unluhizarci, F Kelestimur

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引用次数: 0

Abstract

Purpose: Traumatic Brain Injury (TBI) poses a significant global health burden, with Mild TBI (mTBI) being the most prevalent form. TBI triggers activation of the hypothalamic-pituitary-adrenal (HPA) axis, which in turn affects the hypothalamic-pituitary-gonadal (HPG) axis regulating oogenesis and spermatogenesis. In this study, we investigated the impact of mTBI on sperm genome integrity using a repetitive mTBI (r-mTBI) mouse model.

Methods: We assessed sperm telomere length (TL), free TERRA (fTERRA), and DNA/RNA hybrid TERRA (hTERRA) levels, alongside transcriptional changes in genes involved in TERRA regulation and DNA damage response.

Results: Our findings reveal that a single mTBI event leads to a significant reduction in sperm TL during the acute phase, followed by an increase in TL during the chronic phase of r-mTBI, reminiscent of aging-associated changes. Moreover, we observed alterations in the transcription levels of Rad51, Exo1, Rb1, RNaseH1, and RNaseH2 genes, particularly in association with fTERRA and hTERRA levels, following mTBI.

Conclusion: Understanding the potential non-Mendelian effects of TBI holds promise for elucidating TBI pathogenesis, mechanisms of TBI-induced diseases, and conditions of unknown etiology. Given the risks associated with repeated TBI exposure, especially in sports like football and boxing, consideration of potential paternal transmission of effects to offspring is crucial.

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来源期刊

Journal of Endocrinological Investigation ENDOCRINOLOGY & METABOLISM-

CiteScore

8.10

自引率

7.40%

发文量

242

期刊介绍： The Journal of Endocrinological Investigation is a well-established, e-only endocrine journal founded 36 years ago in 1978. It is the official journal of the Italian Society of Endocrinology (SIE), established in 1964. Other Italian societies in the endocrinology and metabolism field are affiliated to the journal: Italian Society of Andrology and Sexual Medicine, Italian Society of Obesity, Italian Society of Pediatric Endocrinology and Diabetology, Clinical Endocrinologists’ Association, Thyroid Association, Endocrine Surgical Units Association, Italian Society of Pharmacology.