Fission yeast Caprin protein is required for efficient heterochromatin establishment.

IF 4 2区 生物学 Q1 GENETICS & HEREDITY
PLoS Genetics Pub Date : 2025-03-10 eCollection Date: 2025-03-01 DOI:10.1371/journal.pgen.1011620
Haidao Zhang, Ekaterina Kapitonova, Adriana Orrego, Christos Spanos, Joanna Strachan, Elizabeth H Bayne
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引用次数: 0

Abstract

Heterochromatin is a key feature of eukaryotic genomes that serves important regulatory and structural roles in regions such as centromeres. In fission yeast, maintenance of existing heterochromatic domains relies on positive feedback loops involving histone methylation and non-coding RNAs. However, requirements for de novo establishment of heterochromatin are less well understood. Here, through a cross-based assay we have identified a novel factor influencing the efficiency of heterochromatin establishment. We determine that the previously uncharacterised protein is an ortholog of human Caprin1, an RNA-binding protein linked to stress granule formation. We confirm that the fission yeast ortholog, here named Cpn1, also associates with stress granules, and we uncover evidence of interplay between heterochromatin integrity and ribonucleoprotein (RNP) granule formation, with heterochromatin mutants showing reduced granule formation in the presence of stress, but increased granule formation in the absence of stress. We link this to regulation of non-coding heterochromatic transcripts, since in heterochromatin-deficient cells, Cpn1 can be seen to colocalise with accumulating pericentromeric transcripts, and absence of Cpn1 leads to hyperaccumulation of these RNAs at centromeres. Together, our findings unveil a novel link between RNP homeostasis and heterochromatin assembly, and implicate Cpn1 and associated factors in facilitating efficient heterochromatin establishment by enabling removal of excess transcripts that would otherwise impair assembly processes.

裂变酵母的Caprin蛋白是有效建立异染色质所必需的。
异染色质是真核生物基因组的一个关键特征,在着丝粒等区域起着重要的调节和结构作用。在裂变酵母中,现有异色结构域的维持依赖于涉及组蛋白甲基化和非编码rna的正反馈回路。然而,异染色质从头建立的要求尚不清楚。在这里,通过交叉分析,我们已经确定了一个新的因素影响效率的异染色质建立。我们确定先前未表征的蛋白质是人类Caprin1的同源物,Caprin1是一种与应激颗粒形成相关的rna结合蛋白。我们证实,裂变酵母同源物,这里命名为Cpn1,也与压力颗粒有关,我们发现了异染色质完整性和核糖核蛋白(RNP)颗粒形成之间相互作用的证据,异染色质突变体在压力存在时颗粒形成减少,但在没有压力的情况下颗粒形成增加。我们将其与非编码异染色质转录本的调控联系起来,因为在异染色质缺乏的细胞中,可以看到Cpn1与累积的着丝粒周围转录本共定位,并且Cpn1的缺失导致这些rna在着丝粒处的过度积累。总之,我们的研究结果揭示了RNP稳态和异染色质组装之间的新联系,并暗示Cpn1和相关因子通过去除多余的转录本来促进有效的异染色质建立,否则会损害组装过程。
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来源期刊
PLoS Genetics
PLoS Genetics GENETICS & HEREDITY-
自引率
2.20%
发文量
438
期刊介绍: PLOS Genetics is run by an international Editorial Board, headed by the Editors-in-Chief, Greg Barsh (HudsonAlpha Institute of Biotechnology, and Stanford University School of Medicine) and Greg Copenhaver (The University of North Carolina at Chapel Hill). Articles published in PLOS Genetics are archived in PubMed Central and cited in PubMed.
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