Soluble alpha-klotho and 25-hydroxivitamin D are not associated with brown adipose tissue metabolism in young healthy adults.

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of physiology and biochemistry Pub Date : 2025-03-11 DOI:10.1007/s13105-025-01072-z

Francisco J Amaro-Gahete, Héctor Vázquez-Lorente, Guillermo Sanchez-Delgado, Jonatan R Ruiz

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引用次数: 0

Abstract

Background: Soluble Alpha-Klotho (S-αklotho) protein and 25-Hydroxyvitamin D (25-OH-D) have emerged as potential modulators for activating and recruiting Brown Adipose Tissue (BAT). The present study aimed to investigate whether circulating S-αklotho and 25-OH-D levels are related to BAT volume, ¹⁸Fluorine-Fluorodeoxyglucose (¹⁸F-FDG) uptake, and BAT radiodensity in young healthy adults.

Methods: A total of 128 participants (68% women) aged 18-25 years old participated in this cross-sectional study. Serum levels of S-αklotho were determined by a solid-phase sandwich enzyme-linked immunosorbent assay kit and 25-OH-D serum levels were analyzed using a competitive chemiluminescence immunoassay, both in blood samples collected after an overnight fast. All participants underwent a personalized cold exposure to determine their BAT volume, ¹⁸F-FDG uptake, and radiodensity, using a static positron emission tomography combined with computed tomography scan.

Results: After adjusting for multiple covariates, serum levels of S-αklotho (all R² ≤ 0.228 and P ≥ 0.364), 25-OH-D as continuous (all R² ≤ 0.242 and P ≥ 0.088) or by vitamin D status (all R² ≤ 0.767 and P ≥ 0.061) were not associated with either BAT volume and ¹⁸F-FDG uptake, or BAT radiodensity.

Conclusion: Serum S-αklotho and 25-OH-D levels within the physiological range are not related to BAT-related variables in young healthy adults. Further studies are needed to fully understand the underlying mechanisms involved in BAT metabolism in humans. (ACTIBATE; ClinicalTrials.gov identifier: not applicable).

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在年轻健康成人中，可溶性α -克罗索和25-羟基维生素D与棕色脂肪组织代谢无关。

背景：可溶性α - klotho （S-αklotho）蛋白和25-羟基维生素D （25-OH-D）已被发现是激活和募集棕色脂肪组织（BAT）的潜在调节剂。本研究旨在探讨健康青年循环S-α -氯氧托和25-OH-D水平是否与BAT体积、18氟-氟脱氧葡萄糖（18F-FDG）摄取和BAT放射密度有关。方法：共有128名年龄在18-25岁的参与者（68%为女性）参与了这项横断面研究。采用固相三明治酶联免疫吸附测定试剂盒检测血清S-α - klotho水平，采用竞争性化学发光免疫分析法分析血清25-OH-D水平，均为禁食过夜后采集的血液样本。所有参与者都进行了个性化的冷暴露，以确定他们的BAT体积，18F-FDG摄取和辐射密度，使用静态正电子发射断层扫描结合计算机断层扫描。结果：在调整多个协变量后，血清S-α - klotho （R2≤0.228,P≥0.364）、25-OH-D （R2≤0.242,P≥0.088）或维生素D状态（R2≤0.767,P≥0.061）水平与BAT体积和18F-FDG摄取或BAT放射密度均无相关性。结论：健康青壮年血清S-αklotho和25-OH-D水平在生理范围内与bat相关变量无关。需要进一步的研究来充分了解人类BAT代谢的潜在机制。(ACTIBATE;ClinicalTrials.gov标识符：不适用)。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of physiology and biochemistry 生物-生化与分子生物学

CiteScore

6.60

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The Journal of Physiology and Biochemistry publishes original research articles and reviews describing relevant new observations on molecular, biochemical and cellular mechanisms involved in human physiology. All areas of the physiology are covered. Special emphasis is placed on the integration of those levels in the whole-organism. The Journal of Physiology and Biochemistry also welcomes articles on molecular nutrition and metabolism studies, and works related to the genomic or proteomic bases of the physiological functions. Descriptive manuscripts about physiological/biochemical processes or clinical manuscripts will not be considered. The journal will not accept manuscripts testing effects of animal or plant extracts.