Non-CG DNA methylation represses SDC expression to modulate hypocotyl elongation during thermormorphogenesis.

IF 5.6 2区 生物学 Q1 PLANT SCIENCES
Maián Garro, Eleonora Greco, Gustavo J Vannay, Aleksandra Leonova, Leonardo Bruno, Matías Capella
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引用次数: 0

Abstract

Plants adapt to warm environments through physiological and morphological changes termed thermomorphogenesis, which involve transcriptional reprogramming exerted mainly by PHYTOCHROME INTERACTING FACTOR 4 (PIF4). Fluctuating temperatures can also influence the patterns of cytosine DNA methylation, thereby influencing gene expression. However, whether these epigenetic changes provide an adaptative advantage remains unclear. Here, we provide evidence that DNA methylation is required to regulate thermomorphogenesis. Hypomethylated drm1 drm2 cmt3 mutants or seedlings treated with 5-azacytidine to block DNA methylation exhibit reduced hypocotyl growth at warm temperatures, primarily due to impaired cell elongation. Moreover, DNA hypomethylation compromises auxin biosynthesis and transport in response to warmth, partially by reducing PIF4 protein levels. Notably, the loss of DNA methylation leads to increased expression of SUPPRESSOR OF drm1 drm2 cmt3 (SDC), which in turn restricts hypocotyl elongation during thermomorphogenesis. Finally, we demonstrate that DNAme regulates the inhibition of SDC expression to promote gibberellin biosynthesis. Our findings underscore the critical role of DNA methylation in modulating gene expression in response to temperature fluctuations and provide new insights into the epigenetic regulation of thermomorphogenesis.

在热形态形成过程中,非cg DNA甲基化抑制SDC表达以调节下胚轴伸长。
植物通过被称为热形态发生的生理和形态变化来适应温暖的环境,这主要涉及光敏色素相互作用因子4 (PIF4)的转录重编程。温度的波动也会影响胞嘧啶DNA甲基化的模式,从而影响基因的表达。然而,这些表观遗传变化是否提供适应性优势仍不清楚。在这里,我们提供的证据表明,DNA甲基化是调节热形态发生所必需的。低甲基化的drm1 drm2 cmt3突变体或用5-氮胞苷处理以阻断DNA甲基化的幼苗在温暖温度下表现出下胚轴生长减少,主要是由于细胞伸长受损。此外,DNA低甲基化会影响生长素的生物合成和运输,部分原因是PIF4蛋白水平的降低。值得注意的是,DNA甲基化的缺失导致drm1 drm2 cmt3抑制因子(SDC)的表达增加,这反过来限制了热形态形成过程中下胚轴的伸长。最后,我们证明DNAme调节SDC表达抑制,促进赤霉素的生物合成。我们的研究结果强调了DNA甲基化在温度波动下调节基因表达的关键作用,并为热形态发生的表观遗传调控提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Experimental Botany
Journal of Experimental Botany 生物-植物科学
CiteScore
12.30
自引率
4.30%
发文量
450
审稿时长
1.9 months
期刊介绍: The Journal of Experimental Botany publishes high-quality primary research and review papers in the plant sciences. These papers cover a range of disciplines from molecular and cellular physiology and biochemistry through whole plant physiology to community physiology. Full-length primary papers should contribute to our understanding of how plants develop and function, and should provide new insights into biological processes. The journal will not publish purely descriptive papers or papers that report a well-known process in a species in which the process has not been identified previously. Articles should be concise and generally limited to 10 printed pages.
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