{"title":"Clinical Characteristics, Virulence Profile, and Molecular Epidemiology of <i>Klebsiella pneumoniae</i> Infections in Kidney Transplant Recipients.","authors":"Ruiling Fan, Yan Zuo, Bowei Wang, Debao Shi, Xuanxuan Wang, Zhongxin Wang, Ying Huang","doi":"10.2147/IDR.S506794","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong><i>C</i>arbapenem-resistant <i>Klebsiella pneumoniae</i> (CRKP) infections may increase the potential for mortality in kidney transplant (KT) recipients. This study aimed to investigate the clinical features, molecular epidemiology, virulence, and antimicrobial resistance of KP strains from KT patients.</p><p><strong>Methods: </strong>Strains isolated from KT patients were collected, and antimicrobial susceptibility analysis was verified <i>via</i> the Vitek2 compact instrument and the disc diffusion method. In gene expression analysis, carbapenemase genes (KPC-2, OXA-48, IMP, VIM, NDM), capsular genes (K1, K2, K5, K20, K54, K57), and virulence genes (rmpA, rmpA2, aerobactin, peg344) were identified <i>via</i> polymerase chain reaction (PCR). Molecular epidemiology was analyzed using multilocus sequence typing (MLST) and minimal spanning trees (MST).</p><p><strong>Results: </strong>A total of 43 KP isolates were collected from KT patients in this study, and 24 of them were identified as CRKP (55.81%). KPC-2 genes were detected in all of the CRKP strains (100%), and other carbapenemase genes were not detected. Twenty-two strains (91.67%) of CRKP strains were identified as ST11, while 2 (8.33%) were ST15-typing. Finally, two highly virulent <i>K. pneumoniae</i> strains (both K20-ST268 type) were identified. In addition, the group of CRKP showed a higher deceased kidney donor ratio (<i>p</i> = 0.011), a higher proportion of post-transplant transfers to the ICU (<i>p</i> = 0.037), a higher proportion of late-onset infections (3 months post-transplantation acceptance) (p = 0.007), and high positive rates for the virulence gene <i>rmpA2</i> (<i>p</i> = 0.01) when comparing the group of carbapenem-sensitive KP.</p><p><strong>Conclusion: </strong>The resistance rate to carbapenem of KP from KT patients exceeded the regional average with predominant ST typing of ST11. Clinical data were analyzed to derive some high-risk factors for CRKP infection. Therefore, we recommend early prophylactic isolation of transplant patients with high-risk factors for CRKP infection to improve the quality of nosocomial control.</p>","PeriodicalId":13577,"journal":{"name":"Infection and Drug Resistance","volume":"18 ","pages":"1303-1311"},"PeriodicalIF":2.9000,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11892498/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Infection and Drug Resistance","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2147/IDR.S506794","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Carbapenem-resistant Klebsiella pneumoniae (CRKP) infections may increase the potential for mortality in kidney transplant (KT) recipients. This study aimed to investigate the clinical features, molecular epidemiology, virulence, and antimicrobial resistance of KP strains from KT patients.
Methods: Strains isolated from KT patients were collected, and antimicrobial susceptibility analysis was verified via the Vitek2 compact instrument and the disc diffusion method. In gene expression analysis, carbapenemase genes (KPC-2, OXA-48, IMP, VIM, NDM), capsular genes (K1, K2, K5, K20, K54, K57), and virulence genes (rmpA, rmpA2, aerobactin, peg344) were identified via polymerase chain reaction (PCR). Molecular epidemiology was analyzed using multilocus sequence typing (MLST) and minimal spanning trees (MST).
Results: A total of 43 KP isolates were collected from KT patients in this study, and 24 of them were identified as CRKP (55.81%). KPC-2 genes were detected in all of the CRKP strains (100%), and other carbapenemase genes were not detected. Twenty-two strains (91.67%) of CRKP strains were identified as ST11, while 2 (8.33%) were ST15-typing. Finally, two highly virulent K. pneumoniae strains (both K20-ST268 type) were identified. In addition, the group of CRKP showed a higher deceased kidney donor ratio (p = 0.011), a higher proportion of post-transplant transfers to the ICU (p = 0.037), a higher proportion of late-onset infections (3 months post-transplantation acceptance) (p = 0.007), and high positive rates for the virulence gene rmpA2 (p = 0.01) when comparing the group of carbapenem-sensitive KP.
Conclusion: The resistance rate to carbapenem of KP from KT patients exceeded the regional average with predominant ST typing of ST11. Clinical data were analyzed to derive some high-risk factors for CRKP infection. Therefore, we recommend early prophylactic isolation of transplant patients with high-risk factors for CRKP infection to improve the quality of nosocomial control.
期刊介绍：
About Journal
Editors
Peer Reviewers
Articles
Article Publishing Charges
Aims and Scope
Call For Papers
ISSN: 1178-6973
Editor-in-Chief: Professor Suresh Antony
An international, peer-reviewed, open access journal that focuses on the optimal treatment of infection (bacterial, fungal and viral) and the development and institution of preventative strategies to minimize the development and spread of resistance.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
