Pelomonas baiyunensis sp. nov., Pelomonas candidula sp. nov., Pelomonas dachongensis sp. nov., Pelomonas lactea sp. nov., Pelomonas margarita sp. nov., Pelomonas nitida sp. nov., Pelomonas parva sp. nov. and Roseateles rivi sp. nov., isolated from streams in China.

IF 2 3区生物学 Q4 MICROBIOLOGY

International journal of systematic and evolutionary microbiology Pub Date : 2025-03-01 DOI:10.1099/ijsem.0.006707

Huibin Lu, Guannan Li, Shan Yu

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引用次数: 0

Abstract

Eight Gram-stain-negative, aerobic and motile strains (BYS78W^T, BYS87W^T, BYS96W^T, BYS180W^T, DC23W^T, DXS20W^T, LKC17W^T and LYH14W^T) were isolated from streams in China and showed the highest 16S rRNA gene sequence similarities with the species of genus Pelomonas. The calculated average nucleotide identity, digital DNA-DNA hybridization and average amino acid sequence identity values among these eight strains and other closely related strains were less than 89.8, 39.1 and 89.4 %, respectively, indicating that each of the eight strains should represent an independent novel species. The further reconstructed phylogenomic tree showed that seven isolated strains clustered closely with the Pelomonas strains. Only strain BYS180W^T clustered with the Roseateles strains. The phylogenomic tree also showed that the taxonomic status of the recently published species (Paucibacter sediminis, Roseateles aquae, 'Roseateles caseinilyticus', 'Roseateles cellulosilyticus', Roseateles subflavus and Roseateles violae) was questionable. 'R. caseinilyticus' and 'R. cellulosilyticus' should be reassigned to the genus Pelomonas as Pelomonas caseinilytica P7^T (=KACC 22504^T=TBRC 15694^T) and Pelomonas cellulosilytica P8^T (=KACC 22505^T=TBRC 15695^T), respectively. P. sediminis, R. aquae, R. subflavus and R. violae should be reassigned to the genus Kinneretia as Kinneretia sediminis, Kinneretia aquae, Kinneretia subflava and Kinneretia violae, respectively. Combining the above descriptions, strains BYS78W^T, BYS87W^T, BYS96W^T, DC23W^T, DXS20W^T, LKC17W^T and LYH14W^T should represent seven novel species of the genus Pelomonas, for which the names Pelomonas baiyunensis sp. nov. (type strain BYS87W^T=GDMCC 1.4090^T=KCTC 8151^T), Pelomonas candidula sp. nov. (type strain BYS78W^T=GDMCC 1.4089^T=KCTC 8152^T), Pelomonas dachongensis sp. nov. (type strain DC23W^T=GDMCC 1.4087^T=KCTC 8148^T), Pelomonas lactea sp. nov. (type strain DXS20W^T=GDMCC 1.4088^T=KCTC 8147^T), Pelomonas margarita sp. nov. (type strain LKC17W^T=GDMCC 1.4086^T=KCTC 8146^T), Pelomonas nitida sp. nov. (type strain BYS96W^T=GDMCC 1.4091^T=KCTC 8150^T) and Pelomonas parva sp. nov. (type strain LYH14W^T=GDMCC 1.4276^T=KCTC 8301^T) are proposed, respectively. Strain BYS180W^T should represent a novel species of the genus Roseateles, for which the name Roseateles rivi sp. nov. (type strain BYS180W^T=GDMCC 1.4098^T=KCTC 8149^T) is proposed.

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从中国分离到8株革兰氏阴性、需氧和活动菌株（BYS78WT、BYS87WT、BYS96WT、BYS180WT、DC23WT、DXS20WT、LKC17WT和LYH14WT），其16S rRNA基因序列与Pelomonas属的种相似度最高。8株与其他近缘菌株的计算平均核苷酸同源性、数字DNA-DNA杂交和平均氨基酸序列同源性值分别小于89.8、39.1和89.4%，表明8株应分别代表一个独立的新种。进一步重建的系统基因组树显示，7株分离菌株与Pelomonas菌株紧密聚集。只有菌株BYS180WT与蔷薇属菌株聚集在一起。系统基因组树还显示，最近公布的物种（沉淀Paucibacter sediminis， Roseateles aquae, 'Roseateles caseinilyticus', 'Roseateles cellulosilyticus'， Roseateles subflavus和Roseateles violae）的分类地位值得怀疑。‘R. caseinilyticus’和‘R. cellulosilyticus’应重新归属于Pelomonas Pelomonas caseinilytica P7T （=KACC 22504T=TBRC 15694T）和Pelomonas cellulosilytica P8T （=KACC 22505T=TBRC 15695T）。泥沙沙貂、水沙沙貂、黄沙沙貂和紫沙貂应重新划归泥沙沙貂属，分别为泥沙沙貂、水沙沙貂、黄沙沙貂和紫沙沙貂。综上所述，菌株BYS78WT、BYS87WT、BYS96WT、DC23WT、DXS20WT、LKC17WT和LYH14WT应代表7个Pelomonas属新种，其中11月Pelomonas baiyunensis sp. 11（型菌株BYS87WT=GDMCC 1.4090T=KCTC 8151T）、11月Pelomonas dachongensis sp. 11（型菌株BYS78WT=GDMCC 1.4089T=KCTC 8152T）、11月Pelomonas dachongensis sp. 11（型菌株DC23WT=GDMCC 1.4087T=KCTC 8148T）、11月Pelomonas lactea sp. 11（型菌株DXS20WT=GDMCC 1.4088T=KCTC 8147T）、提出了玛格丽塔褐单胞菌（类型菌株LKC17WT=GDMCC 1.4086T=KCTC 8146T）、褐藻褐单胞菌（类型菌株BYS96WT=GDMCC 1.4091T=KCTC 8150T）和小褐单胞菌（类型菌株LYH14WT=GDMCC 1.4276T=KCTC 8301T）。菌株BYS180WT为玫瑰属新种，建议将其命名为Roseateles rivi sp. nov（类型菌株BYS180WT=GDMCC 1.4098T=KCTC 8149T）。

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来源期刊

International journal of systematic and evolutionary microbiology 生物-微生物学

CiteScore

5.20

自引率

21.40%

发文量

426

审稿时长

1 months

期刊介绍： Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.