Cartilaginous fishes-derived chondroitin sulfates potentially suppress lipid droplet accumulation in the differentiated 3T3-L1 adipocytes.

IF 2.7 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Glycoconjugate Journal Pub Date : 2025-04-01 Epub Date: 2025-03-10 DOI:10.1007/s10719-025-10183-0
Danang Dwi Cahyadi, Katsuhiko Warita, Naoko Takeda-Okuda, Jun-Ichi Tamura, Yoshinao Z Hosaka
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引用次数: 0

Abstract

In this study, we investigated for cell proliferative and adipogenic differentiation inhibitory activities of chondroitin sulfate (CS) from cartilaginous fish: mako shark (Isurus oxyrinchus, spine part, Ms-CS), blue shark (Prionace glauca, spine part, Bs-CS), sharpspine skate (Okamejei acutispina, head and tail parts, Sp-CS) and stingray (Dasyatis akajei, head part, St-CS) on 3T3-L1 cells. Most of the CSs from cartilaginous fish showed concentration-dependent cell proliferative activity of 3T3-L1 cells within the retrieved concentration range (0-1,000 μg/mL), while under induction of adipocyte differentiation, they inhibited lipid accumulation. In particular, Ms-CS and Sp-CS were highly active in inhibiting lipid accumulation in the cells. The present study revealed that cartilaginous fish-derived CS has inhibitory activity on 3T3-L1 adipocyte differentiation by suppressing lipid droplet accumulation, although the degree of suppression varied depending on the composition of the CS and its origin. In addition, a significant increase in chondroitin sulfate N-acetylgalactosaminyltransferase 2 (Csgalnact2) expression of the Sp-CS group at the concentration of 500 µg/mL was observed. Csgalnact2 expression is associated with chondroitin N-acetylgalactosaminyltransferase-2 (ChGn-2), one of the glycosyltransferases that catalyzes the chain initiation and elongation of the CS backbone in its biosynthesis. Exogenous CS from cartilaginous fishes increased Csgalnact2 expression, although further studies are needed to confirm changes in CS biosynthesis. We observed reduced lipid accumulation in differentiated 3T3-L1 cells. Our findings highlight the role of CS polysaccharides, in inhibiting adipogenesis, even though further investigation is required to understand the underlying mechanism.

软骨鱼来源的硫酸软骨素可能抑制分化的3T3-L1脂肪细胞中的脂滴积累。
本研究以灰鲭鲨(Isurus oxyrinchus,脊柱部分,Ms-CS)、青鲨(Prionace glauca,脊柱部分,Bs-CS)、尖刺鳐(Okamejei acutispina,头尾部分,Sp-CS)和黄貂鱼(Dasyatis akajei,头部部分,St-CS)为研究对象,研究了硫酸软骨素(CS)对3T3-L1细胞的增殖和成脂分化抑制作用。在提取的浓度范围(0 ~ 1000 μg/mL)内,大多数软骨鱼的CSs对3T3-L1细胞的增殖活性呈浓度依赖性,而在诱导脂肪细胞分化的作用下,它们能抑制脂质积累。其中Ms-CS和Sp-CS在抑制细胞脂质积累方面表现出较高的活性。本研究发现,软骨鱼源CS通过抑制脂滴积累对3T3-L1脂肪细胞分化具有抑制活性,尽管抑制程度因CS的组成和来源而异。此外,Sp-CS组在500µg/mL浓度下,硫酸软骨素n -乙酰半乳糖氨基转移酶2 (Csgalnact2)表达显著升高。Csgalnact2的表达与软骨素n-乙酰半乳糖氨基转移酶-2 (ChGn-2)有关,ChGn-2是一种糖基转移酶,在其生物合成中催化CS主链的起始和延伸。来自软骨鱼类的外源CS增加了Csgalnact2的表达,尽管还需要进一步的研究来证实CS生物合成的变化。我们观察到分化的3T3-L1细胞中脂质积累减少。我们的研究结果强调了CS多糖在抑制脂肪形成方面的作用,尽管需要进一步的研究来了解其潜在的机制。
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来源期刊
Glycoconjugate Journal
Glycoconjugate Journal 生物-生化与分子生物学
CiteScore
6.00
自引率
3.30%
发文量
63
审稿时长
1 months
期刊介绍: Glycoconjugate Journal publishes articles and reviews on all areas concerned with: function, composition, structure, biosynthesis, degradation, interactions, recognition and chemo-enzymatic synthesis of glycoconjugates (glycoproteins, glycolipids, oligosaccharides, polysaccharides and proteoglycans), biochemistry, molecular biology, biotechnology, immunology and cell biology of glycoconjugates, aspects related to disease processes (immunological, inflammatory, arthritic infections, metabolic disorders, malignancy, neurological disorders), structural and functional glycomics, glycoimmunology, glycovaccines, organic synthesis of glycoconjugates and the development of methodologies if biologically relevant, glycosylation changes in disease if focused on either the discovery of a novel disease marker or the improved understanding of some basic pathological mechanism, articles on the effects of toxicological agents (alcohol, tobacco, narcotics, environmental agents) on glycosylation, and the use of glycotherapeutics. Glycoconjugate Journal is the official journal of the International Glycoconjugate Organization, which is responsible for organizing the biennial International Symposia on Glycoconjugates.
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