Polyvalent immunoprotection of protein, DNA and IgY antibody vaccines of Vibrio fluvialis outer membrane protein VF08100 in fish.

IF 4.1 2区农林科学 Q1 FISHERIES

Fish & shellfish immunology Pub Date : 2025-03-08 DOI:10.1016/j.fsi.2025.110260

Huihui Xiao, Saixing Duan, Pan Cui, Jing Chen, Xixian Che, Juan Lu, Juan Wang, Guoping Zhu, Yong Liu, Xiang Liu

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引用次数: 0

Abstract

Vaccines are of great importance to green aquaculture. In this study, the polyvalent immunoprotective activities of protein, DNA and egg yolk antibody (IgY) vaccines of Vibrio fluvialis outer membrane protein VF08100 were assessed. Carassius auratus was immunized with these three vaccines and challenged with V. fluvialis and Aeromonas hydrophila. The results showed that the three vaccines had significant immune protection rates (p < 0.01) against V. fluvialis and A. hydrophila infections, could activate the congenital immune response in C. auratus, and downregulate the expression of inflammation genes (p < 0.01) and antioxidant factors (p < 0.01) to reduce the inflammatory response and antioxidant reactions, respectively. Moreover, the three vaccines could protect the internal tissue structure integrity and reduce the apoptosis and DNA damage of kidney cells induced by bacterial infection. Therefore, the protein, DNA and IgY vaccines of VF08100 resisted multiple bacterial infections and can be used as polyvalent candidate vaccines for aquaculture.

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来源期刊

Fish & shellfish immunology 农林科学-海洋与淡水生物学

CiteScore

7.50

自引率

19.10%

发文量

750

审稿时长

68 days

期刊介绍： Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.