Comparison of the newly formulated beckman access free T4 (Thyroxine) assay on the DXI 800 analyzer with the current Beckman free T4 (Thyroxine) assay for biotin Interference: New assay is free from biotin interference

IF 3.2 3区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Clinica Chimica Acta Pub Date : 2025-03-08 DOI:10.1016/j.cca.2025.120239

Kameron Pierce, Bailey Countryman, Melody Nelson, Amitava Dasgupta

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引用次数: 0

Abstract

Background

Biotin causes significant positive interference with Free T4 (thyroxine) measurement using the current Beckman assay. Recently, Beckman reformulated the FT4 assay to overcome biotin interference. We compared the effect of biotin on both current and newly formulated FT4 assays.

Materials and Methods

Three serum pools were prepared using specimens containing various amounts of FT4. Aliquots of each pool were supplemented with various amounts of biotin. Then FT4 concentrations were measured by both the current and new FT4 assay. In addition, three volunteers ingested 10 mg of biotin. Specimens were drawn prior to the administration of biotin and 2 h post-biotin ingestion. FT4 concentration was measured before and after biotin administration using both assays.

Results

The newly formulated FT4 assay correlated well with the current FT4 assay. In the presence of biotin, FT4 concentrations were increased significantly using the current assay. However, negligible changes were observed using the newly formulated assay. We also observed similar results in vivo within the volunteer specimens.

Conclusion

The newly formulated Beckman Access FT4 assay is free from biotin interference up to a very high biotin concentration of 3500 ng/mL.

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来源期刊

Clinica Chimica Acta 医学-医学实验技术

CiteScore

10.10

自引率

2.00%

发文量

1268

审稿时长

23 days

期刊介绍： The Official Journal of the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) Clinica Chimica Acta is a high-quality journal which publishes original Research Communications in the field of clinical chemistry and laboratory medicine, defined as the diagnostic application of chemistry, biochemistry, immunochemistry, biochemical aspects of hematology, toxicology, and molecular biology to the study of human disease in body fluids and cells. The objective of the journal is to publish novel information leading to a better understanding of biological mechanisms of human diseases, their prevention, diagnosis, and patient management. Reports of an applied clinical character are also welcome. Papers concerned with normal metabolic processes or with constituents of normal cells or body fluids, such as reports of experimental or clinical studies in animals, are only considered when they are clearly and directly relevant to human disease. Evaluation of commercial products have a low priority for publication, unless they are novel or represent a technological breakthrough. Studies dealing with effects of drugs and natural products and studies dealing with the redox status in various diseases are not within the journal''s scope. Development and evaluation of novel analytical methodologies where applicable to diagnostic clinical chemistry and laboratory medicine, including point-of-care testing, and topics on laboratory management and informatics will also be considered. Studies focused on emerging diagnostic technologies and (big) data analysis procedures including digitalization, mobile Health, and artificial Intelligence applied to Laboratory Medicine are also of interest.