Saponins from sea cucumber disrupt Aeromonas hydrophila quorum sensing to mitigate pathogenicity.

IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Banafsheh Payam, Mehdi Soltani, Mehdi Shamsaie Mehrgan, Houman Rajabi Islami, Melika Nazemi
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Abstract

Aeromonas hydrophila, a Gram-negative bacterium, poses significant threats to aquaculture, leading to substantial economic losses. Its pathogenicity is primarily driven by a sophisticated quorum sensing (QS) system that regulates virulence factors. This study investigates saponins extracted from the sea cucumber Holothuria leucospilota as potential natural inhibitors of QS in A. hydrophila, offering a novel disease management strategy for aquaculture. Specimens of H. leucospilota were collected, and saponins were extracted from their Cuvierian tubules through a process of homogenization, solvent extraction, and purification. The saponin extract's minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) against A. hydrophila were found to be 80 μg/mL and 30 μg/mL, respectively. Hemolysin, lipase, and protease are examples of QS-regulated virulence factors whose activities were found to be significantly reduced by sub-MIC levels of saponins. Additionally, swarming motility and biofilm formation were notably inhibited. A significant downregulation of the QS genes ahyI and ahyR was observed, indicating an effective disruption of the QS system.These findings suggest that saponins from H. leucospilota can inhibit the QS system in A. hydrophila, thereby reducing its pathogenicity. This disruption offers a promising method for controlling bacterial infections without directly killing the bacteria, potentially mitigating antibiotic resistance. The study highlights the potential of marine-derived compounds as natural QS inhibitors, contributing to environmentally friendly aquaculture practices. Overall, it provides evidence that sea cucumber saponins could serve as a novel class of anti-QS agents, presenting a new perspective on disease management in aquaculture and other bacterial infection contexts.

海参皂苷破坏嗜水气单胞菌群体感应以减轻致病性。
嗜水气单胞菌是一种革兰氏阴性菌,对水产养殖构成重大威胁,造成重大经济损失。其致病性主要由一个复杂的群体感应(QS)系统驱动,该系统调节毒力因子。本研究研究了海参Holothuria leucospilota中提取的皂苷对嗜水藻(a.s hydrophila) QS的天然抑制作用,为水产养殖提供了一种新的疾病管理策略。采集白花蓟马标本,通过均质、溶剂萃取、纯化等工艺从其居维氏小管中提取皂苷。皂苷提取物对嗜水单胞菌的最低杀菌浓度(MBC)为80 μg/mL,最低抑菌浓度(MIC)为30 μg/mL。溶血素、脂肪酶和蛋白酶是qs调节的毒力因子的例子,它们的活性被发现被亚mic水平的皂苷显著降低。此外,蜂群运动和生物膜的形成也明显受到抑制。QS基因ahyI和ahyR显著下调,表明QS系统受到有效破坏。这些结果表明,白刺草皂苷可以抑制嗜水单胞菌的QS系统,从而降低其致病性。这种破坏提供了一种很有前途的方法,可以在不直接杀死细菌的情况下控制细菌感染,从而有可能减轻抗生素耐药性。该研究强调了海洋衍生化合物作为天然QS抑制剂的潜力,有助于环境友好型水产养殖实践。综上所示,海参皂苷可作为一类新的抗qs药物,为水产养殖和其他细菌感染环境下的疾病管理提供了新的视角。
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来源期刊
AMB Express
AMB Express BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
7.20
自引率
2.70%
发文量
141
审稿时长
13 weeks
期刊介绍: AMB Express is a high quality journal that brings together research in the area of Applied and Industrial Microbiology with a particular interest in ''White Biotechnology'' and ''Red Biotechnology''. The emphasis is on processes employing microorganisms, eukaryotic cell cultures or enzymes for the biosynthesis, transformation and degradation of compounds. This includes fine and bulk chemicals, polymeric compounds and enzymes or other proteins. Downstream processes are also considered. Integrated processes combining biochemical and chemical processes are also published.
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