Precious I Richard, William H Baltosser, Philip H Williams, Qingfang He
{"title":"Phylogenetic analysis of microbial CP-lyase cluster genes for bioremediation of phosphonate.","authors":"Precious I Richard, William H Baltosser, Philip H Williams, Qingfang He","doi":"10.1186/s13568-025-01856-y","DOIUrl":null,"url":null,"abstract":"<p><p>The ever-increasing use of phosphonates and their derivatives has resulted in the discharge of large quantities of these materials into the ecosystem, causing pollution and harmful shifts in microbiome composition. We conducted an extensive phylogenetic analysis to address this mounting problem and to help determine suitable microbes for bioremediation in specific environments. The 84 microorganisms included in our study span the gamut of species and occupied habitats. They degrade phosphonates by expressing an enzyme complex; CP-Lyase transcribed from 14 cistrons. Of the organisms studied, 12, 39, and 25 are singularly suitable for mostly freshwater, marine, or terrestrial habitats, respectively. Others adapted to multihabitats include Calothrix sp. PCC 7507 (both freshwater and marine habitats), Escherichia coli, Kaistia soli, Limoniibacter endophyticus, Marivita sp. and Virgibacillus dokdonensis (both marine and terrestrial habitats), Acidithiobacillus ferrooxidans (both freshwater and terrestrial habitats), with Paenibacillus contaminans suitable for freshwater, marine, and terrestrial habitats. All organisms were statistically rooted to glutathione peroxidase for phylogenetic perspective with tree topology dependent upon 50% or greater support. Clustered genes have been shown to have co-evolved based on striking nucleotide similarity and clade groupings within the tree topologies generated.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"42"},"PeriodicalIF":3.5000,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11893972/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"AMB Express","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1186/s13568-025-01856-y","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The ever-increasing use of phosphonates and their derivatives has resulted in the discharge of large quantities of these materials into the ecosystem, causing pollution and harmful shifts in microbiome composition. We conducted an extensive phylogenetic analysis to address this mounting problem and to help determine suitable microbes for bioremediation in specific environments. The 84 microorganisms included in our study span the gamut of species and occupied habitats. They degrade phosphonates by expressing an enzyme complex; CP-Lyase transcribed from 14 cistrons. Of the organisms studied, 12, 39, and 25 are singularly suitable for mostly freshwater, marine, or terrestrial habitats, respectively. Others adapted to multihabitats include Calothrix sp. PCC 7507 (both freshwater and marine habitats), Escherichia coli, Kaistia soli, Limoniibacter endophyticus, Marivita sp. and Virgibacillus dokdonensis (both marine and terrestrial habitats), Acidithiobacillus ferrooxidans (both freshwater and terrestrial habitats), with Paenibacillus contaminans suitable for freshwater, marine, and terrestrial habitats. All organisms were statistically rooted to glutathione peroxidase for phylogenetic perspective with tree topology dependent upon 50% or greater support. Clustered genes have been shown to have co-evolved based on striking nucleotide similarity and clade groupings within the tree topologies generated.
期刊介绍:
AMB Express is a high quality journal that brings together research in the area of Applied and Industrial Microbiology with a particular interest in ''White Biotechnology'' and ''Red Biotechnology''. The emphasis is on processes employing microorganisms, eukaryotic cell cultures or enzymes for the biosynthesis, transformation and degradation of compounds. This includes fine and bulk chemicals, polymeric compounds and enzymes or other proteins. Downstream processes are also considered. Integrated processes combining biochemical and chemical processes are also published.