The Effect of Heparin on Bone Metabolism and Orthodontic Tooth Movement in Rats

IF 1.7 Q3 DENTISTRY, ORAL SURGERY & MEDICINE
Behzad Salari, Reza Moradian, Yasaman Kheirandish, Mojgan Alaeddini, Shahroo Etemad-Moghadam, Shahla Maghsoudi, Ahmad Reza Dehpour
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引用次数: 0

Abstract

Objectives

Various attempts have been made to increase the rate of orthodontic tooth movement (OTM). The aim of this study was to determine the effect of different doses of heparin on OTM and paraclinical factors related to bone metabolism in rats.

Methods and Materials

A total of 24 Sprague-Dawley rats were randomly divided into three groups of 8 animals each and injected with 0 (control), 3000, and 6000 U/Kg/d heparin sulfate for 4 weeks. Radiographs were obtained at the initiation and at the end of the study period. Orthodontic forces were applied on Day 14 and continued for the next 2 weeks, after which, OTM, optical density, parathyroid hormone (PTH) level, and histologic variables were assessed for each rat. The latter was performed on hematoxylin/eosin–stained sections of the mesial roots of the first molar and included calculation of the osteoclast number, and resorption lacunae depth and number. One-way analysis of variance, the Tukey test, and a paired-t-test were used for statistical analysis (p < 0.05).

Results

A significant increase in OTM, the number of resorptive lacunae, and PTH secretion was observed in the group that received 6000 U/Kg/d compared with both the other groups. There was no significant difference in optical density, and, therefore, bone density, among the study groups (p > 0.05).

Conclusion

Heparin injection affects bone metabolism in rats, as shown by the increases in OTM and PTH and its impact on histologic parameters. These effects seem to be dose-dependent and may be a factor that should be taken into consideration during orthodontic treatment planning.

Abstract Image

肝素对大鼠骨代谢及正畸牙齿运动的影响
目的为了提高正畸牙齿的移动率,人们进行了各种各样的尝试。本研究旨在探讨不同剂量肝素对大鼠骨代谢相关的OTM及临床旁因子的影响。方法与材料选取24只Sprague-Dawley大鼠,随机分为3组,每组8只,分别注射0(对照)、3000、6000 U/Kg/d硫酸肝素4周。在研究开始时和研究结束时拍摄x线片。在第14天施加正畸力并持续2周,之后评估每只大鼠的OTM、光密度、甲状旁腺激素(PTH)水平和组织学指标。后者是在苏木精/伊红染色的第一磨牙近根切片上进行的,包括计算破骨细胞数量、吸收陷窝深度和数量。统计学分析采用单因素方差分析、Tukey检验和配对t检验(p < 0.05)。结果与其他两组相比,6000 U/Kg/d组的OTM、吸收腔隙数量和甲状旁腺激素分泌明显增加。各研究组之间光密度无显著差异,因此骨密度也无显著差异(p > 0.05)。结论肝素注射对大鼠骨代谢有影响,表现为OTM和PTH的升高及其对组织学参数的影响。这些影响似乎是剂量依赖性的,可能是在正畸治疗计划中应该考虑的一个因素。
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来源期刊
Clinical and Experimental Dental Research
Clinical and Experimental Dental Research DENTISTRY, ORAL SURGERY & MEDICINE-
CiteScore
3.30
自引率
5.60%
发文量
165
审稿时长
26 weeks
期刊介绍: Clinical and Experimental Dental Research aims to provide open access peer-reviewed publications of high scientific quality representing original clinical, diagnostic or experimental work within all disciplines and fields of oral medicine and dentistry. The scope of Clinical and Experimental Dental Research comprises original research material on the anatomy, physiology and pathology of oro-facial, oro-pharyngeal and maxillofacial tissues, and functions and dysfunctions within the stomatognathic system, and the epidemiology, aetiology, prevention, diagnosis, prognosis and therapy of diseases and conditions that have an effect on the homeostasis of the mouth, jaws, and closely associated structures, as well as the healing and regeneration and the clinical aspects of replacement of hard and soft tissues with biomaterials, and the rehabilitation of stomatognathic functions. Studies that bring new knowledge on how to advance health on the individual or public health levels, including interactions between oral and general health and ill-health are welcome.
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