The GmMYB1-GmbHLHA-GmCPC-like module regulates light-induced anthocyanin production in soybean sprouts

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-03-06 DOI:10.1016/j.plaphy.2025.109738

Mengyang Niu , Youyou Guo , Gang Hu , Liuyi Li , Yaping Lu , Yanwu Lu , Xingxing Yuan , Zhenguo Shen , Nana Su

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引用次数: 0

Abstract

Soybean (Glycine max L.) is an important economic crop, flavonoids (such as anthocyanins) and some other nutrients of which were significantly promoted after germination. The accumulation of anthocyanin is influenced by many kinds of factors in plants, the regulatory mechanism of which is relatively complex. Here, soybean double mutant stf1/2 was utilized and found that GmSTF1/2 participated in light-mediated anthocyanin production in soybean. GmMYB1 was considered as a direct target of GmSTF1/2. Expressing GmMYB1 in soybean hair roots and tobacco significantly promoted anthocyanin content. GmMYB1 could directly bind to the promoters of GmDFR, GmANS, and GmUFGT, thereby promoting their transcriptions. In addition, GmMYB1 interacted with GmbHLHA, and their interaction could enhance the functions of GmMYB1 in positively regulating anthocyanin accumulation. R3-MYB GmCPC-like was activated by GmMYB1 when anthocyanin was abundant. Expressing GmCPC-like significantly inhibited anthocyanin contents in soybean hair roots and tobacco. GmCPC-like inhibited anthocyanin accumulation mainly through interacting with GmMYB1 and GmbHLHA, and then decreased their positive roles in anthocyanin production. Taken together, the GmMYB1-GmbHLHA-GmCPC-like module finely regulates anthocyanin production in soybean sprouts.

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.