Plastidial metabolites and retrograde signaling: A case study of MEP pathway intermediate MEcPP that orchestrates plant growth and stress responses

Abstract

Plants are frequently exposed to environmental stresses. In a plant cell, chloroplast acts as machinery that rapidly senses changing environmental conditions and coordinates with the nucleus and other subcellular organelles by exchanging plastidial metabolites, proteins/peptides, or lipid derivatives, some of which may act as retrograde signals. These specific plastidial metabolites include carotenoid derivatives, isoprenes, phosphoadenosines, tetrapyrroles, phytohormone (like salicylic acid), and reactive electrophile species (RES), which mediate retrograde communications to sustain stress conditions. The methylerythritol phosphate (MEP) pathway is an essential and evolutionarily conserved isoprenoid biosynthetic pathway operating in bacteria and plastids, synthesizing metabolites such as terpenoids, gibberellins, abscisic acid, phytol chain of chlorophyll, carotenoids, tocopherols, and glycosides. The MEP pathway is susceptible to oxidative stress, which results in the overaccumulation of its intermediates, such as methylerythritol cyclodiphosphate (MEcPP). Recent studies revealed that under stress conditions, leading to its accumulation, MEcPP mediates retrograde signaling that alters the nuclear gene expression, leading to growth inhibition and acclimation. This review covers aspects of its generation, signaling, mechanism of action, and interplay with other factors to acquire adaptive responses during stress conditions. The review highlights the importance of plastids as sensors of stress and plastidial metabolites as retrograde signals communicating with nucleus and other sub-cellular organelles to regulate plants’ response to different stress conditions.