Nanopore toward Genuine Single-Molecule Sensing: Molecular Ping-Pong Technology

IF 9.1 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Letters Pub Date : 2025-02-26 DOI:10.1021/acs.nanolett.4c0608510.1021/acs.nanolett.4c06085

Xinjia Zhao, Yahui Zhang and Guangyan Qing*,

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Abstract

Nanopore sensing is a so-called label-free, single-molecule technology; however, multiple events of different molecules are recorded to obtain statistically robust data, which can limit both efficiency and sample use. To overcome these challenges, nanopore molecular ping-pong technology enables precise single-molecule manipulation, reducing systematic and stochastic errors by repeatedly measuring the same molecule. This review introduces the fundamentals and advancements of ping-pong technology, highlighting a recent breakthrough achieving over 10,000 recaptures of a single dsDNA molecule within minutes. This innovation not only minimizes sample requirements, which is critical for nonamplifiable samples, but also significantly enhances experimental precision. While current applications focus on dsDNA, extending this technology to protein and glycan analysis could transform nanopore research. Just as nanopore technology revolutionized DNA sequencing, it holds the potential to drive the development of nanopore-based protein and glycan sequencers, paving the way for groundbreaking advancements in molecular biology and biomedicine.

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纳米孔走向真正的单分子传感：分子乒乓技术

纳米孔传感是一种所谓的无标签单分子技术；然而，记录不同分子的多个事件以获得统计上可靠的数据，这可能会限制效率和样本使用。为了克服这些挑战，纳米孔分子乒乓技术实现了精确的单分子操作，通过重复测量同一分子来减少系统和随机误差。这篇综述介绍了乒乓球技术的基本原理和进展，重点介绍了最近的一项突破，即在几分钟内重新捕获单个dsDNA分子超过10,000次。这一创新不仅最大限度地减少了样品需求，这对不可放大的样品至关重要，而且显著提高了实验精度。虽然目前的应用主要集中在双链dna上，但将这项技术扩展到蛋白质和聚糖分析可能会改变纳米孔的研究。正如纳米孔技术彻底改变了DNA测序一样，它也有可能推动纳米孔蛋白和聚糖测序仪的发展，为分子生物学和生物医学的突破性进展铺平道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nano Letters 工程技术-材料科学：综合

CiteScore

16.80

自引率

2.80%

发文量

1182

审稿时长

1.4 months

期刊介绍： Nano Letters serves as a dynamic platform for promptly disseminating original results in fundamental, applied, and emerging research across all facets of nanoscience and nanotechnology. A pivotal criterion for inclusion within Nano Letters is the convergence of at least two different areas or disciplines, ensuring a rich interdisciplinary scope. The journal is dedicated to fostering exploration in diverse areas, including: - Experimental and theoretical findings on physical, chemical, and biological phenomena at the nanoscale - Synthesis, characterization, and processing of organic, inorganic, polymer, and hybrid nanomaterials through physical, chemical, and biological methodologies - Modeling and simulation of synthetic, assembly, and interaction processes - Realization of integrated nanostructures and nano-engineered devices exhibiting advanced performance - Applications of nanoscale materials in living and environmental systems Nano Letters is committed to advancing and showcasing groundbreaking research that intersects various domains, fostering innovation and collaboration in the ever-evolving field of nanoscience and nanotechnology.