Regulating the hydrophobic part in amphiphilic triphenylamino-diarylethene fluorophores for ultrasensitive and portable detection of urinary albumin

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-03-11 DOI:10.1016/j.snb.2025.137592

Caiqiang Liao, Xianwen Liang, Tianyi Qin, Xinfeng Du, Haoyan Xiong, Quan Chen, Cuizhen Lu, Zhongyang Zhang, Zhongyong Xu, Taoyuze Lv, Zhaohui Wang, Yalong Wang, Bin Liu, Mingqiang Zhu

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引用次数: 0

Abstract

Sensitive and accurate detection of urinary albumin (u-ALB), preferably by user-friendly smartphone-based portable devices, is essential for the prevention and regular evaluation of chronic kidney disease (CKD). Here, we present a class of ionic aggregation-induced emission fluorogens (AIEgens) containing triphenylamine pyridinium skeleton and extended alkyl chains (TPA-Ps) as novel probes for u-ALB. Different from the previous studies focusing on regulating the number of ionic moieties, we for the first time investigated the effect of extending the hydrophobic tails on enhancing the sensitivity. Specifically, the TPA-P-C6 with one ionic moiety yet a n-hexyl chain exhibited the highest fluorescent increasing fold (> 3450) on responding to u-ALB, which has also been the highest degree among the reported ionic AIEgen-based u-ALB probes. Different stages of albuminuria (A1-A3) represented by varying u-ALB concentrations could be clearly revealed through fluorescence spectral signal readout. Additionally, the TPA-P-C6 exhibited a restored fluorescence at longer wavelength area in real urine samples, enabling fluorocolorimetric sensing and point-of-care testing by naked eye. With the assistance of smartphones for signal capturing and analysis, portable devices based on test papers, 96-well plate, and 3D-printing technique have been constructed for the on-site detection of u-ALB. Compared with the commercial dye based u-ALB indicator (G250) and test papers, the multi-color sensing signals of our probe can clearly reveal the different stages of albuminuria and offer useful proofs for early diagnosis of CKD.

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.