Molecular mechanisms by which polyethylene terephthalate (PET) microplastic and PET leachate promote the growth of benthic cyanobacteria

IF 11.4 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Water Research Pub Date : 2025-03-10 DOI:10.1016/j.watres.2025.123476

Aonan Yang, Haiyan Pei, Ming Zhang, Yan Jin, Hangzhou Xu

{"title":"Molecular mechanisms by which polyethylene terephthalate (PET) microplastic and PET leachate promote the growth of benthic cyanobacteria","authors":"Aonan Yang, Haiyan Pei, Ming Zhang, Yan Jin, Hangzhou Xu","doi":"10.1016/j.watres.2025.123476","DOIUrl":null,"url":null,"abstract":"Toxic blooms of benthic cyanobacteria greatly threaten freshwater ecological health and drinking water safety. Meanwhile, microplastic pollution is becoming increasingly severe and microplastics accumulate in large quantities at the bottom of lakes and rivers, widely coexisting with algae. However, impacts of microplastics on benthic cyanobacteria are still unknown. This study investigated effects of microplastic polyethylene terephthalate (PET) — which is commonly found at the bottom of lakes and rivers — and its leachate at environmentally relevant concentration (0.3 mg/L) and high exposure concentration (3.0 mg/L) on typical benthic cyanobacteria (<em>Oscillatoria</em> sp. and <em>Pseudanabaena</em> sp.), and clarified the related molecular mechanisms through transcriptomic analysis. Results show that PET or PET leachate (PET-L) can promote benthic cyanobacterial growth and promotive effect of PET-L is more obvious than that of PET system. Promotion effect of PET or PET-L is more significant at environmentally relevant concentration (39–63% increase compared with the control) compared with high exposure concentration (21–58% increase compared with the control). In the presence of PET or PET-L, due to an increase in the number of cyanobacterial cells, concentrations of harmful metabolites (cylindrospermopsin, geosmin, and 2-methylisoborneol) in water also increased. Although PET particles may not be conducive to benthic cyanobacterial growth due to shading effect and mechanical damage, photosynthetic efficiency of algae was improved and dysregulated genes related to photosynthesis and extracellular transport of glycolipid were upregulated according to transcriptome analysis. Moreover, PET decomposition components, such as terephthalic acid and ethylene glycol, may be able to serve as carbon sources for cyanobacterial growth. Upregulation of genes associated with glycolysis, oxidative phosphorylation, and translation revealed that PET can promote the growth of benthic cyanobacteria. This study has important value in evaluating the impact of benthic cyanobacteria on aquatic ecological health and drinking water safety with the coexistence of microplastics.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"86 1","pages":""},"PeriodicalIF":11.4000,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Water Research","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1016/j.watres.2025.123476","RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ENVIRONMENTAL","Score":null,"Total":0}

引用次数: 0

Abstract

Toxic blooms of benthic cyanobacteria greatly threaten freshwater ecological health and drinking water safety. Meanwhile, microplastic pollution is becoming increasingly severe and microplastics accumulate in large quantities at the bottom of lakes and rivers, widely coexisting with algae. However, impacts of microplastics on benthic cyanobacteria are still unknown. This study investigated effects of microplastic polyethylene terephthalate (PET) — which is commonly found at the bottom of lakes and rivers — and its leachate at environmentally relevant concentration (0.3 mg/L) and high exposure concentration (3.0 mg/L) on typical benthic cyanobacteria (Oscillatoria sp. and Pseudanabaena sp.), and clarified the related molecular mechanisms through transcriptomic analysis. Results show that PET or PET leachate (PET-L) can promote benthic cyanobacterial growth and promotive effect of PET-L is more obvious than that of PET system. Promotion effect of PET or PET-L is more significant at environmentally relevant concentration (39–63% increase compared with the control) compared with high exposure concentration (21–58% increase compared with the control). In the presence of PET or PET-L, due to an increase in the number of cyanobacterial cells, concentrations of harmful metabolites (cylindrospermopsin, geosmin, and 2-methylisoborneol) in water also increased. Although PET particles may not be conducive to benthic cyanobacterial growth due to shading effect and mechanical damage, photosynthetic efficiency of algae was improved and dysregulated genes related to photosynthesis and extracellular transport of glycolipid were upregulated according to transcriptome analysis. Moreover, PET decomposition components, such as terephthalic acid and ethylene glycol, may be able to serve as carbon sources for cyanobacterial growth. Upregulation of genes associated with glycolysis, oxidative phosphorylation, and translation revealed that PET can promote the growth of benthic cyanobacteria. This study has important value in evaluating the impact of benthic cyanobacteria on aquatic ecological health and drinking water safety with the coexistence of microplastics.

Abstract Image

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Water Research 环境科学-工程：环境

CiteScore

20.80

自引率

9.40%

发文量

1307

审稿时长

38 days

期刊介绍： Water Research, along with its open access companion journal Water Research X, serves as a platform for publishing original research papers covering various aspects of the science and technology related to the anthropogenic water cycle, water quality, and its management worldwide. The audience targeted by the journal comprises biologists, chemical engineers, chemists, civil engineers, environmental engineers, limnologists, and microbiologists. The scope of the journal include: •Treatment processes for water and wastewaters (municipal, agricultural, industrial, and on-site treatment), including resource recovery and residuals management; •Urban hydrology including sewer systems, stormwater management, and green infrastructure; •Drinking water treatment and distribution; •Potable and non-potable water reuse; •Sanitation, public health, and risk assessment; •Anaerobic digestion, solid and hazardous waste management, including source characterization and the effects and control of leachates and gaseous emissions; •Contaminants (chemical, microbial, anthropogenic particles such as nanoparticles or microplastics) and related water quality sensing, monitoring, fate, and assessment; •Anthropogenic impacts on inland, tidal, coastal and urban waters, focusing on surface and ground waters, and point and non-point sources of pollution; •Environmental restoration, linked to surface water, groundwater and groundwater remediation; •Analysis of the interfaces between sediments and water, and between water and atmosphere, focusing specifically on anthropogenic impacts; •Mathematical modelling, systems analysis, machine learning, and beneficial use of big data related to the anthropogenic water cycle; •Socio-economic, policy, and regulations studies.