Honghao Xu, Hongfei Liu, Ye Liu, Wenhua Guo, Yue Zhao, Haoze Ying, Yaru Liu, Zhe Li, Yi Zhang, Lei Tao, Tuya Siqin, Wa Gao, Xiangling You
{"title":"Over-expression of AeWRKY2 promotes oleanolic acid and hederagenin accumulation in Aralia elata.","authors":"Honghao Xu, Hongfei Liu, Ye Liu, Wenhua Guo, Yue Zhao, Haoze Ying, Yaru Liu, Zhe Li, Yi Zhang, Lei Tao, Tuya Siqin, Wa Gao, Xiangling You","doi":"10.1016/j.biochi.2025.03.001","DOIUrl":null,"url":null,"abstract":"<p><p>Oleanolic acid (OA) and hederagenin from Aralia elata have important medicinal value due to their anti-cancer and anti-inflammatory activities. Transcription factors (TFs) can affect the production of active substances by regulating the expression of genes in metabolic pathways. In this study, a transcription factor AeWRKY2 was cloned and overexpressed in A. elata. The result of gene expression analysis and high-performance liquid chromatography (HPLC) showed that the key enzyme genes of all transgenic strains analyzed were up-regulated to varying degrees. The contents of oleanolic acid and hederagenin were higher than those of wild type, up to 2.773 mg·g<sup>-1</sup> DW and 0.325 mg·g<sup>-1</sup> DW, respectively. After one-day MeJA treatment, the contents of oleanolic acid and hederagenin were further increased to 3.31 mg·g<sup>-1</sup> and 0.37 mg·g<sup>-1</sup>, respectively.</p>","PeriodicalId":93898,"journal":{"name":"Biochimie","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimie","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.biochi.2025.03.001","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Oleanolic acid (OA) and hederagenin from Aralia elata have important medicinal value due to their anti-cancer and anti-inflammatory activities. Transcription factors (TFs) can affect the production of active substances by regulating the expression of genes in metabolic pathways. In this study, a transcription factor AeWRKY2 was cloned and overexpressed in A. elata. The result of gene expression analysis and high-performance liquid chromatography (HPLC) showed that the key enzyme genes of all transgenic strains analyzed were up-regulated to varying degrees. The contents of oleanolic acid and hederagenin were higher than those of wild type, up to 2.773 mg·g-1 DW and 0.325 mg·g-1 DW, respectively. After one-day MeJA treatment, the contents of oleanolic acid and hederagenin were further increased to 3.31 mg·g-1 and 0.37 mg·g-1, respectively.
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