Over-expression of AeWRKY2 promotes oleanolic acid and hederagenin accumulation in Aralia elata.

Honghao Xu, Hongfei Liu, Ye Liu, Wenhua Guo, Yue Zhao, Haoze Ying, Yaru Liu, Zhe Li, Yi Zhang, Lei Tao, Tuya Siqin, Wa Gao, Xiangling You
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Abstract

Oleanolic acid (OA) and hederagenin from Aralia elata have important medicinal value due to their anti-cancer and anti-inflammatory activities. Transcription factors (TFs) can affect the production of active substances by regulating the expression of genes in metabolic pathways. In this study, a transcription factor AeWRKY2 was cloned and overexpressed in A. elata. The result of gene expression analysis and high-performance liquid chromatography (HPLC) showed that the key enzyme genes of all transgenic strains analyzed were up-regulated to varying degrees. The contents of oleanolic acid and hederagenin were higher than those of wild type, up to 2.773 mg·g-1 DW and 0.325 mg·g-1 DW, respectively. After one-day MeJA treatment, the contents of oleanolic acid and hederagenin were further increased to 3.31 mg·g-1 and 0.37 mg·g-1, respectively.

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