Over-expression of AeWRKY2 promotes oleanolic acid and hederagenin accumulation in Aralia elata

IF 3.3 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochimie Pub Date : 2025-03-05 DOI:10.1016/j.biochi.2025.03.001

Honghao Xu , Hongfei Liu , Ye Liu , Wenhua Guo , Yue Zhao , Haoze Ying , Yaru Liu , Zhe Li , Yi Zhang , Lei Tao , Tuya Siqin , Wa Gao , Xiangling You

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Abstract

Oleanolic acid (OA) and hederagenin from Aralia elata have important medicinal value due to their anti-cancer and anti-inflammatory activities. Transcription factors (TFs) can affect the production of active substances by regulating the expression of genes in metabolic pathways. In this study, a transcription factor AeWRKY2 was cloned and overexpressed in A. elata. The result of gene expression analysis and high-performance liquid chromatography (HPLC) showed that the key enzyme genes of all transgenic strains analyzed were up-regulated to varying degrees. The contents of oleanolic acid and hederagenin were higher than those of wild type, up to 2.773 mg g⁻¹ DW and 0.325 mg g⁻¹ DW, respectively. After one-day MeJA treatment, the contents of oleanolic acid and hederagenin were further increased to 3.31 mg g⁻¹ and 0.37 mg g⁻¹, respectively.

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AeWRKY2的过表达促进了木本植物齐墩果酸和hederagenin的积累。

楤木中的齐墩果酸（OA）和hederagenin具有抗癌和抗炎作用，具有重要的药用价值。转录因子可以通过调节代谢途径中基因的表达来影响活性物质的产生。本研究克隆了一个转录因子AeWRKY2，并在elata中过表达。基因表达分析和高效液相色谱分析结果显示，所有转基因菌株的关键酶基因均有不同程度的上调。齐墩果酸和hederagenin的含量均高于野生型，分别为2.773 mg·g-1 DW和0.325 mg·g-1 DW。MeJA处理1天后，齐墩果酸和hederenin的含量分别增加到3.31 mg·g-1和0.37 mg·g-1。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biochimie 生物-生化与分子生物学

CiteScore

7.20

自引率

2.60%

发文量

219

审稿时长

40 days

期刊介绍： Biochimie publishes original research articles, short communications, review articles, graphical reviews, mini-reviews, and hypotheses in the broad areas of biology, including biochemistry, enzymology, molecular and cell biology, metabolic regulation, genetics, immunology, microbiology, structural biology, genomics, proteomics, and molecular mechanisms of disease. Biochimie publishes exclusively in English. Articles are subject to peer review, and must satisfy the requirements of originality, high scientific integrity and general interest to a broad range of readers. Submissions that are judged to be of sound scientific and technical quality but do not fully satisfy the requirements for publication in Biochimie may benefit from a transfer service to a more suitable journal within the same subject area.