{"title":"Effects of traditional Chinese medicine pyritum on orthodontic tooth movement in rats.","authors":"Zhuoying Zhang, Jingwei Wang, Chuanqi Hua, Yanpeng Zhang, Wanyu Xi, Ruofang Zhang","doi":"10.1016/j.ajodo.2025.02.003","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>The objective of this study was to investigate the effects of pyritum on tooth movement.</p><p><strong>Methods: </strong>Thirty 9-week-old male Sprague Dawley rats were divided into 3 groups: control group, tooth movement + saline (TM+S) group, and tooth movement + pyritum (TM+P) group. The OTM model was established in both the TM+S group and the TM+P group. TM+P group received a local injection of pyritum decoction, whereas the other 2 groups received normal saline. Tooth movement distance was measured after 7 and 14 days. Histologic stains included hematoxylin and eosin staining for tissue morphology, tartrate-resistant acid phosphatase staining for osteoclasts, and immunohistochemistry for receptor activator of nuclear factor kappa β ligand (RANKL), osteoprotegerin (OPG), runt-related protein 2, and 5-bromo-2'-dexoyuridine.</p><p><strong>Results: </strong>At day 14, local injection of pyritum significantly (P <0.05) slowed down tooth movement, with the number of osteoclasts in the TM+P group significantly (P <0.05) reduced than that in the TM+S group. Runt-related protein 2 demonstrated higher expression in the TM+P group than in the TM+S group on both pressure and tension sides (P <0.05). The number of 5-bromo-2'-dexoyuridine-positive cells on the tension side in the TM+P group was significantly (P <0.05) higher than that in the TM+S group. RANKL expression on the pressure side in the TM+P group was significantly (P <0.05) lower than that in the TM+S group, whereas OPG expression exhibited an opposite trend. On days 7 and 14, the RANKL/OPG ratio in the TM+P group was significantly (P <0.05) lower than that in the TM+S group.</p><p><strong>Conclusions: </strong>The study indicated that pyritum was capable of slowing down the speed of tooth movement while potentially promoting bone formation and inhibiting bone resorption.</p>","PeriodicalId":50806,"journal":{"name":"American Journal of Orthodontics and Dentofacial Orthopedics","volume":" ","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"American Journal of Orthodontics and Dentofacial Orthopedics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.ajodo.2025.02.003","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
引用次数: 0
Abstract
Introduction: The objective of this study was to investigate the effects of pyritum on tooth movement.
Methods: Thirty 9-week-old male Sprague Dawley rats were divided into 3 groups: control group, tooth movement + saline (TM+S) group, and tooth movement + pyritum (TM+P) group. The OTM model was established in both the TM+S group and the TM+P group. TM+P group received a local injection of pyritum decoction, whereas the other 2 groups received normal saline. Tooth movement distance was measured after 7 and 14 days. Histologic stains included hematoxylin and eosin staining for tissue morphology, tartrate-resistant acid phosphatase staining for osteoclasts, and immunohistochemistry for receptor activator of nuclear factor kappa β ligand (RANKL), osteoprotegerin (OPG), runt-related protein 2, and 5-bromo-2'-dexoyuridine.
Results: At day 14, local injection of pyritum significantly (P <0.05) slowed down tooth movement, with the number of osteoclasts in the TM+P group significantly (P <0.05) reduced than that in the TM+S group. Runt-related protein 2 demonstrated higher expression in the TM+P group than in the TM+S group on both pressure and tension sides (P <0.05). The number of 5-bromo-2'-dexoyuridine-positive cells on the tension side in the TM+P group was significantly (P <0.05) higher than that in the TM+S group. RANKL expression on the pressure side in the TM+P group was significantly (P <0.05) lower than that in the TM+S group, whereas OPG expression exhibited an opposite trend. On days 7 and 14, the RANKL/OPG ratio in the TM+P group was significantly (P <0.05) lower than that in the TM+S group.
Conclusions: The study indicated that pyritum was capable of slowing down the speed of tooth movement while potentially promoting bone formation and inhibiting bone resorption.
期刊介绍：
Published for more than 100 years, the American Journal of Orthodontics and Dentofacial Orthopedics remains the leading orthodontic resource. It is the official publication of the American Association of Orthodontists, its constituent societies, the American Board of Orthodontics, and the College of Diplomates of the American Board of Orthodontics. Each month its readers have access to original peer-reviewed articles that examine all phases of orthodontic treatment. Illustrated throughout, the publication includes tables, color photographs, and statistical data. Coverage includes successful diagnostic procedures, imaging techniques, bracket and archwire materials, extraction and impaction concerns, orthognathic surgery, TMJ disorders, removable appliances, and adult therapy.
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